Comparison of Neomycin Production from Streptomyces fradiae Cultivation Using Soybean Oil as the Sole Carbon Source in an Air-Lift Bioreactor and a Stirred-Tank Reactor
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概要
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Streptomyces fradiae was cultivated in both an air-lift bioreactor and a jar-fermentor with various agitation rates from 200 to 800 rpm to investigate differences in neomycin production between the two reactors. Final neomycin concentrations in the jar-fermentor operated at 600 rpm and the air-lift bioreactor were 3.19 and 1.39 g/l, respectively. On the other hand, levels of soybean oil consumption in the two reactors were 25.9 and 9.4 g/l, respectively. Shear stress due to mechanical agitation caused changes in the morphology of mycelia and influenced neomycin production. The morphological changes of the mycelia in the jar-fermentor caused the viscosity of the culture broth decrease by half, and soybean oil consumption and fatty acid uptake rate to increase 3- and 1.8-fold, respectively, in comparison with those of the air-lift bioreactor. The product yield coefficient determined from the level of soybean oil consumption in the air-lift bioreactor was similar to that of the jar-fermentor at 600 rpm, but the neomycin yield was less than one-half. In the case of the jar-fermentor, the yield increased with increasing agitation rate and was maximum at 600 rpm. To maximize neomycin production in S. fradiae cultures using soybean oil as sole carbon source, it was neccessary to provide a degree of shear stress to the mycelia and to optimize liquid mixing.In an air-lift bioreactor, the soybean oil consumption may be suppressed due to a low dgree of liquid mixing.
- 社団法人日本生物工学会の論文
- 1995-05-25
著者
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Park Y
Shizuoka Univ. Shizuoka Jpn
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Park Yong
Biochemical Engineering Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizu
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Park Yong
(present Address)department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Universi
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Park Yong
Biochemical Eng. Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Uni
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OKABE MITSUYASU
Department of Applied Biological Chemistry, Faculty of agriculture, Shizuoka University
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OKABE MITSUYASU
Biochemical Engineering Laboratory, Department of Applied Biological Chemistry, Faculty of Agricultu
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Okabe Mitsuyasu
Biochemical Eng. Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Uni
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Okabe Mitsuyasu
Mercian Co. Central Research Laboratories
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Okabe Mitsuyasu
Biotechnology Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Univer
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Okabe Mitsuyasu
Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University
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Okabe Mitsuyasu
Biochemical Engineering Laboratory Department Of Applied Biological Chemistry Faculty Of Agriculture
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Okabe Mitsuyasu
(present Address)department Of Applied Biochemistry Faculty Of Agriculture Shizuoka University
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Ohta N
Kumamoto Univ. Kumamoto Jpn
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Ohta Noriyasu
Biochemical Engineering Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizu
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Okabe M
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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YAHIRO KAZUTOYO
United Graduate School of Agricultural Science, Gifu University
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Yahiro Kazutoyo
Department Of Development Chikou Co. Ltd.
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Ohta N
Engineering Department Suntory Limited
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