A Novel Process of Cyclodextrin Production by the Use of Specific Adsorbents : Part II. A New Reactor System for Selective Production of α-Cyclodextrin with Specific Adsorbent

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概要

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• We have developed a novel process of α-cyclodextrin(α-CD)production by using a new adsorbent that is characterized by its exceedingly powerful selectivity for α-CD compared with other CDs. α-CD production was carried out in a closed reactor system that was composed of a main reactor, wherein liquefied starch was converted to CDs by cyclodextrin glucosyltransferase(CGTase;EC2.4.1.19), and a column packed with the adsorbent. While the reaction mixture was circulated in the system, α-CD was selectively adsorbed in the column and its concentration in the mixture of the main reactor was kept at a low level. This low concentration of α-CD stimulated the conversion of starch to CDs and as a result, enhanced its yield based on added starch. When 8.3%(w/v)of liquefied starch was used in the reactor system, the yield of α-CD was 22.2% and α-CD occuied 58.7% of the reaction mixture of total CDs synthesized. Meanwhile, in a batch system without the adsorbent, the yield of α-CD and its fraction were 10.8% and 45.0%, respectively. After the conversion reaction, and following the preliminary washing with water through the column, α-CD was easily eluted with hot water, resulting in a high purity of about 95%.

• 社団法人日本生物工学会の論文
• 1991-06-25

著者

• Nomura Hideo

  Mercian Co. Central Research Laboratories

• OKABE MITSUYASU

  Department of Applied Biological Chemistry, Faculty of agriculture, Shizuoka University

• Okabe Mitsuyasu

  Mercian Co. Central Research Laboratories

• Okabe Mitsuyasu

  Biotechnology Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Univer

• Okabe Mitsuyasu

  Laboratory Of Biotechnology Faculty Of Agriculture Shizuoka University

• Okabe Mitsuyasu

  Biochemical Engineering Laboratory Department Of Applied Biological Chemistry Faculty Of Agriculture

• Okabe Mitsuyasu

  (present Address)department Of Applied Biochemistry Faculty Of Agriculture Shizuoka University

• Okamoto R

  Mercian Corp. Fujisawa Jpn

• Okabe M

  Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University

• OKAMOTO Rokuro

  Mercian Corporation, Central Research Laboratories

• TSUCHIYAMA YUKIO

  Mercian Co., Central Research Laboratories

• Tsuchiyama Yukio

  Mercian Co. Central Research Laboratories

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