Purification and Characterization of cis-Aconitic Acid Decarboxylase from Aspergillus terreus TN484-M1(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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cis-Aconitic acid decarboxylase (CAD) was assumed to be a key enzyme in the production of itaconic acid by comparing the activity of CAD from Aspergillus terreus TN484-M1 with that of CAD from the low-itaconate yielding strain Aspergillus terreus CM85J. The constitutive CAD was purified to homogeneity from A. terreus TN484-M1 by ammonium sulfate fractionation, and column chromatography on DEAE-toyopearl, Butyl-toyopearl, and Sephacryl S200HR, and then characterized. A molecular mass of 55 kDa for the native enzyme was determined by SDS-PAGE. The enzymic activity was optimal at a pH of 6.2 and temperature of 45℃ The K_m value for cisaconitic acid was determined as 2.45 mM (pH 6.2, 37℃). The enzyme was completely inactivated by Hg^+, Cu^<2+>, Zn^<2+>,p-chloromercuribenzoate, and 5,5'-dithio-bis(2-nitrobenzoate).
- 社団法人日本生物工学会の論文
- 2002-07-25
著者
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OKABE MITSUYASU
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shi
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Kahar Prihardi
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Dwiarti Lies
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Okabe Mitsuyasu
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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Yamane Ken
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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YAMATANI HITOSHI
Department of Genetics, The Graduate University for Advanced Studies, National Institute of Genetics
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Yamatani Hitoshi
Department Of Genetics The Graduate University For Advanced Studies National Institute Of Genetics
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Okabe Mitsuyasu
Laboratory Of Biotechnology Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuo
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