Purification and Characterization of Two Isozymes of Chlorophyllase from Mature Leaves of Chenopodium album

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概要

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• Chlorophyllase (Chlase) was purified from mature leaves of Chenopodium album, and its enzymatic properties were investigated. Chlase was extracted from powder of C. album and purified by the following chromatographic procedures: hydrophobic chromatography, Con A Sepharose, Heparin affinity chromatography, Mono Q ion-exchange chrouratography, and gel-filtration. Con A Sepharose affinity chromatography and gel-filtration were the most effective steps on the purification. On Mono Q chromatography, the Chlase preparation separated into two major and one minor fractions that exhibited Chlase activity. The two major Chlases were purified to homogeneity. Their molecular masses were estimated as 41.3 kDa and 40.2 kDa by SDS-PAGE. The optimum pH and K_m values of these two Chlases were similar. Their N-terminal amino acid sequences were almost identical except for a deletion in the tenth amino acid residue in one of the Chlase; there was no homologous protein detected by database search.

• 日本植物生理学会の論文

著者

• Mikami B

  Kyoto Univ. Kyoto Jpn

• Mikami Bunzo

  Graduate School Of Agriculture Kyoto Univ.

• Mikami Bunzo

  Research Institute For Food Science Kyoto University

• Ohta Hiroyuki

  Tokyo Institute of Technology, Department of Biological Sciences, Faculty of Bioscience and Biotechn

• Masuda Tatsuru

  Tokyo Institute of Technology, Department of Biological Sciences, Faculty of Bioscience and Biotechn

• Shioi Yuzo

  Tokyo Institute of Technology, Department of Biological Sciences, Faculty of Bioscience and Biotechn

• Takamiya Ken-ichiro

  Tokyo Institute of Technology, Department of Biological Sciences, Faculty of Bioscience and Biotechn

• SHIRANO Yumiko

  Mitsui Plant Biotechnology Research Institute

• Shioi Y

  Mitsui Plant Biotechnology Research Institute

• Shioi Yuzo

  Department Of Biological Sciences Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technol

• Ohta Hiroyuki

  Dept. Biol. Sci. Tokyo Inst. Technol.

• Masuda T

  Department Of Biological Sciences Graduate School Of Bioscience And Biotechnology Tokyo Institute Of

• Mikami B

  Kyoto Univ.

• Tsuchiya Tohru

  Tokyo Institute of Technology, Faculty of Bioscience and Biotechnology, Department of Biological Sci

• Kita Noriaki

  Tokyo Institute of Technology, Department of Biological sciences, Faculty of Bioscience and Biotechn

• Kita Noriaki

  Tokyo Institute Of Technology Department Of Biological Sciences Faculty Of Bioscience And Biotechnol

• Tsuchiya T

  Department Of Biological Sciences Graduate School Of Bioscience And Biotechnology Tokyo Institute Of

• Tsuchiya Tohru

  Faculty Of Agriculture Tohoku University

• Mikami Bunzo

  Div. Of Food Sci. And Biotechnology Graduate School Of Agriculture Kyoto Univ.

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