Soybean Lipoxygenase L-4, a Component of the 94-kilodalton Storage Protein in Vegetative Tissues : Expression and Accumulation in Leaves Induced by Pod Removal and by Methyl Jasmonate

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A lipoxygenase L-4 gene was isolated from a soybean genomic library. The amino acid sequence of lipoxygenase L-4 is highly homologous with the partial amino acid sequence of the 94-kDa vegetative storage protein, vsp94, found in paraveinal mesophyll cells in the leaves of depodded soybean plants. No L-4 expression was observed in maturing seeds. The L-4 gene is highly expressed in the vegetative tissues of young seedlings, including cotyledons, hypocotyls, roots and primary leaves. L-4 expression followed the same pattern as lipoxygenase activity in cotyledons peaking 3 to 5 days after germination, and returning to a basal level by 9 days after germination. L-4 gene expression was low in the roots, stems and leaves of 10-week-old plants. Exposure of 4-week-old plants to atmospheric methyl jasmonate increased L-4 mRNA in leaves. Continuous pod removal from 7-week-old plants over a 2 week period resulted in dramatic accumulation of L-4 mRNA in leaves. Accumulation of the L-4 protein and three other lipoxygenase fractions in the leaves of depodded plants was demonstrated by ion exchange chromatography. These results indicate that lipoxygenase L-4 is a component of vsp94.
日本植物生理学会の論文