INDUCTION OF RAT DUODENAL ALKALINE PHOSPHATASE ACTIVITY BY BILE IN VITRO (<Symposium>Histo- and Cytochemistry in Tissue Culture)
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概要
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The effect of the bile, an inductive factor of duodenal alkaline phosphatase (Alpase) in rats with surgically produced bile duct ligation and in organ cultures, was investigated by biochemical and cytochemical methods. Surgical bile duct ligation induced Alpase activity on the duodenal cells and also rats injected with bile from rats with bile duct ligation showed a great increase in activity. Duodenum from 1-month-old rats could best be maintained for 24 hr in a modified millipore filter roller tube using DM-153 medium without serum. Results in organ culture confirmed the observation in vivo and showed the induction of Alpase activity in the duodenum after administration of the bile and the depression of the rate of Alpase induction by the bile under tunicamycin presence. These results suggest that the bile from rats with bile duct ligation contains an inductive factor of Alpase in the duodenal cells. Organ culture is a very useful method and good model for the analysis of inductive factor of some substances in cell or tissues. Our findings are discussed in this paper.
- 日本組織細胞化学会の論文
著者
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Komoda Tsugikazu
Department Of Biochemistry Faculty Of Medicine Saitama Medical University
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KUMEGAWA Masayoshi
Department of Oral Anatomy, Meikai University School of Dentistry
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Yajima Toshihiko
Department Of Oral Anatomy Higashi-nippon-gakuen University School Of Dentistry
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Yajima Toshihiko
Department Of Dental Radiology School Of Dentistry Health Sciences University Of Hokkaido
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Kumegawa Masayoshi
Department Of Oral Anatomy Josai Dental University
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SAKAGISHI YOSHIKATSU
Department of Biochemistry, Saitama Medical School
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Komoda Tsugikazu
Department Of Biochemistry Saitama Medical School
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Sakagishi Yoshikatsu
Department Of Biochemistry Saitama Medical School
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