<ORIGINAL ARTICLE>Tartrate-resistant acid phosphatase activity induced by pre-incubation with tartrate in mouse embryonic mandibles
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概要
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The present study used mouse embryonic mandibles to examine the characteristics of tartrate in tartrate-resistant acid phosphatase (TR-ACPase) histochemistry. Short-term incubation (30 min) in substrate-containing reaction medium showed intense and specific activity of TR-ACPase only in a small number of mononuclear cells, presumably pre-osteoclasts, present around a population of differentiating osteoblasts. Pre-incubation with tartrate and subsequent incubation of reaction medium resulted in slightly increased intensity in the preosteoclasts and also weak enzyme activity in other cells such as oral and dental epithelia, osteoblasts, and chondrocytes of Meckel's cartilage. Pre-incubation with tartrate and subsequent incubation with reaction medium may result in overestimation of the histochemical products. Therefore short-term incubation is important to estimate the enzyme activity exactly in TR-ACPase histochemistry. especially in osteoblasts.
- 北海道医療大学の論文
- 1997-12-30
著者
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YAJIMA Toshihiko
北海道医療大学歯学部
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Sakakura Yasunori
Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido
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Yajima T
北海道医療大学 口腔解剖
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TSURUGA EICHI
Department of Oral Anatomy, Health Sciences University of Hokkaido School of Dentistry
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SAKAKURA Yasunori
北海道医療大学歯学部
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Yajima Toshihiko
Department Of Dental Radiology School Of Dentistry Health Sciences University Of Hokkaido
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Tsuruga Eichi
Department Of Oral Anatomy Health Sciences University Of Hokkaido School Of Dentistry
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Sakakura Yasunori
Department Of Oral Anatomy School Of Dentistry Health Sciences University Of Hokkaido
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TSURUGA Eichi
北海道医療大学歯学部
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Sakakura Yasunori
Department Of Dental Radiology School Of Dentistry Health Sciences University Of Hokkaido
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Sakakura Y
Department Of Oral Anatomy School Of Dentistry Health Sciences University Of Hokkaido
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SAKAKURA Yasunori/TSURUGA
北海道医療大学歯学部
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