Molecular Characteristics of Porcine Thymus Histone H1 Variants

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概要

• 論文の詳細を見る

• We constructed an assay system to measure the annealing activity that is one of the functional features of nuclear proteins, using ssDNAs derived from M13 phage recombinants which contained a complementary 406-bp portion each. Histone H1 variants were puritied from porcine thymus by separation of chromatin, extraction with 5% perchloric acid, and reversed-phase HPLC. Three types of histone H1 variants were found by analysis of amino acid composition and on SDS-PAGE. All of these could promote the annealing. According to Hill's analysis all had similar numbers of binding sites to DNA strands but dissociation constants and annealing activity were different. The number of binding sites, dissociation constants, and annealing activity were changed by dephosphorylation of histone H1 variants. This result suggests that histone H1 variants have different affinities for DNA molecules and ssDNA-annealing activity, which is regulated by phosphorylation.

• 社団法人日本農芸化学会の論文
• 1995-03-23

著者

• UNO Tomohide

  Department of Applied Bioscience, Faculty of Agriculture, Saga University

• Uno Tomohide

  Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University

• Yasuo Aizono

  Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University

• Toshiaki Uchikawa

  Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

• Teruo Iwasaki

  Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

• Toshiaki Uchikawa

  Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University

• Teruo Iwasaki

  Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe University

関連論文

• Characteristics of Psychrophilic Alkaline Phosphatase
• Molecular Characteristics of Porcine Thymus Histone H1 Variants

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