A Six-well Plate Method: Less Laborious and Effective Method for Cultivation of Obligate Anaerobic Microorganisms

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概要

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• We developed a simple, less laborious method to cultivate and isolate obligate anaerobic microorganisms using a six-well plate together with the AnaeroPack System, designated as the six-well plate method. The cultivation efficiency of this method, based on colony-forming units, colony formation time, and colony size, was evaluated with four authentic obligate anaerobes (two methanogenic archaea and two sulfate-reducing bacteria). The method was found to be comparable to or even better than the roll tube method, a technique that is commonly used at present for the cultivation of obligate anaerobes. Further experiments using 21 representative obligate anaerobes demonstrated that all examined anaerobes (11 methanogens, 5 sulfate- or thiosulfate-reducing bacteria, and 5 syntrophs) could form visible colonies on the six-well plate and that these colonies could be successfully subcultured in fresh liquid media. Using this method, an unidentified sulfate-reducing bacterium was successfully isolated from an environmental sample.

著者

• Kamagata Yoichi

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Tamaki Hideyuki

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Nakamura Kazunori

  Bioproduction Res. Inst. National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• Kang Myung

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Mochimaru Hanako

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (aist)

• NAKAMURA KOHEI

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• Lee Sung-taik

  Department Of Biological Sciences Kaist (korea Advanced Institute Of Science And Technology)

• Mochimaru Hanako

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• KAMAGATA YOICHI

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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