Molecular Analysis of AsamyR Gene Encoding Transcriptional Factor for Amylolytic Gene from Shoyu Koji Mold, Aspergillus sojae KBN1340
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概要
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The gene, designated AsamyR, encoding a transcriptional activator involved in amylolytic gene expression was isolated from a shoyu koji mold, Aspergillus sojae KBN1340, and characterized. The structural gene comprised 1,951 bp with 2 introns. AsAmyR consisted of 595 amino acid residues possessing a high degree of sequence identity with other Aspergillus AmyRs. The AsamyR gene disruptant showed significantly restricted growth on starch agar plates and produced no detectable α-amylase production in SP medium. The multicopy AsamyR strain exhibited approximately two-fold higher amylase activity when compared to that of the control strain, but the increased rate of the amylase activity was not as high as the copy number of the integrated AsamyR gene.
著者
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Kato Masashi
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
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Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
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YOSHINO-YASUDA Shoko
Food Research Center, Aichi Center for Industry and Science Technology
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FUJINO Emi
Food Research Center, Aichi Center for Industry and Science Technology
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MATSUI Junko
Food Research Center, Aichi Center for Industry and Science Technology
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ONO Natsuko
Food Research Center, Aichi Center for Industry and Science Technology
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