Xylose Triggers Reversible Phosphorylation of XlnR, the Fungal Transcriptional Activator of Xylanolytic and Cellulolytic Genes in aspergillus oryzae
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概要
- 論文の詳細を見る
XlnR is a transcription factor that mediates D-xylose-triggered induction of xylanolytic and cellulolytic genes in Aspergillus. In order to clarify the molecular mechanisms underlying XlnR-mediated induction, Aspergillus oryzae XlnR was fused with the c-myc tag and examined by Western blotting. Phosphate-affinity SDS–PAGE revealed that XlnR was present as a mixture of variously phosphorylated forms in the absence of D-xylose, and that D-xylose triggered additional phosphorylation of the protein. D-Xylose-triggered phosphorylation was a rapid process occurring within 5 min prior to the accumulation of xynG2 mRNA, and removal of D-xylose caused slow dephosphorylation, leading to less-phosphorylated forms. At 30 min after removal, the phosphorylation status was almost identical to that in the absence of D-xylose, and the level of xynG2 mRNA started to decrease. These results indicate that XlnR is highly phosphorylated when it is active in transactivation, implying that D-xylose-triggered reversible phosphorylation controls XlnR activity.
- 社団法人 日本農芸化学会の論文
- 2011-05-23
著者
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Kato Masashi
Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University
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Kato Masashi
Department Of Bioenvironmental Medicine Graduate School Of Medicine Chiba University
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Kanamaru Kyoko
Department Of Microbiology And Immunology Institute Of Medical Science University Of Tokyo:(present
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Kobayashi Tetsuo
Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University
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Kato Masashi
Dep. Of Material And Environmental Chemistry Graduate School Of Engineering Utsunomiya Univ.
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Kanamaru Kyoko
Lab. Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Kobayashi Tetsuo
Department Of Anatomy Ii School Of Veterinary Medicine Azabu University
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Kobayashi Tetsuo
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Noguchi Yuji
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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TANAKA Hisaki
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nago
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Tanaka Hisaki
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Kato Masashi
Department Of Applied Biological Chemistry Fuculty Of Agriculture Meijo University
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Kato Masashi
Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University
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Kanamaru Kyoko
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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NOGUCHI Yuji
Department of Applied Chemistry, School of Engineering, The University of Tokyo:(Present address)The Univ. of Tokyo, RCAST Intelligent Materials lab.
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KOBAYASHI Tetsuo
Department of Anatomy II
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