Nucleotide Sequence of an Endo-1,4-β-Glucanase Gene (celA) from Clostridium josui

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概要

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• The nucleotide sequence of a DNA fragment containing and endo-1,4-β-glucanase (EG-1) gene of Clostridium josui was determined by the dideoxy-chain termination method. The EG-1 coding sequence was an open reading frame enocoding 369 amino acids, and a putative promoter sequence was located in the up-stream region of the open reading frame. The N-terminal amino acid sequence of the endoglucanase (EG-1C) purified from the Escherichia coli transformant (JM103/pUCJ1) was consistent with the deduced sequence from ^<30>Val to ^<44>Lys. The estimated molecular weights of the precursor and the mature enzymas were 41,774 and 38,352,respectively. The region of amino acids from 61st to 335th of the enzyme revealed high homology with those of Bacillus sp. and Clostridium acetobutylicum endoglucanases.

• 公益社団法人日本生物工学会の論文
• 1992-04-25

著者

• Fujino Tsuchiyoshi

  Bio Development Division Nagoya Seiraku Co. Ltd.

関連論文

• Antimutagenic Effects of Ajoene, an Organosulfur Compound Derived from Garlic
• Nucleotide Sequence of an Endo-1,4-β-Glucanase Gene (celA) from Clostridium josui
• Cloning of the celB Gene Encoding Endo-1,4-β-Glucanase-2 from Clostridium josui in Escherichia coli and the Properties of the Translated Product

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