Cloning and Expression in Escherichia coli of Thermophilic Clostridium sp. F1 Genes Related to Cellulose Hydrolysis(Microbiology & Fermentation Industry)
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概要
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Eleven distinct fragments of Clostridium sp. stain F1 (which was isolated and identified in this laboratory) DNA have been cloned in E. coli and shown to express enzymatic activities related to cellulose hydrolysis. Six of the 11 E. coli clones showed endoglucanase activity, 2 showed cellobiohy-drolase activity as well as endoglycanase activity, one showed β-glucosidase activity and the 2 others showed exoglucanase activity as well as xylanase activity. On comparing the restriction maps of the plasmids constructed in this study with those of C. thermocellum genes, it was concluded that 7 of the 11 clones carried hitherto unidentified cellulase genes, i.e., 4 endoglucanase genes, 1β-glucosidase gene and 2 xylanase/exoglucanase genes, and that the 4 other clones carried the same genes as those found in C. thermocellum.
- 社団法人日本農芸化学会の論文
- 1989-04-23
著者
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Sakka Kazuo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department Of Bioscience Faculty Of Bioresources Mie University
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Sakka Kazuo
Department Of Bioscience Faculty Of Bioresources Mie University
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Furuse Shinji
Department Of Bioscience Faculty Of Bioresources Mie University:(present Office)ozeki Shuzo Co. Ltd.
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