Gene Cloning, Purification, and Characterization of a Lactate Oxidase from Lactococcus lactis subsp. cremoris IFO3427
スポンサーリンク
概要
- 論文の詳細を見る
Lactococcus lactis subsp. cremoris IFO3427 produced both a well-characterized lactate dehydrogenase and a lactate oxidase. The lactate oxidase gene, designated loxL, was cloned and almost 200-fold overexpressed in Escherichia coli compared to the wild-type strain. The nucleotide sequence of loxL gene, composed of 1, 101 bp and 367 amino acid residues, was also determined. The primary structure of LoxL was highly homologous to those of several α-hydroxyacid oxidases. LoxL was purified from the recombinant strain and characterized. Properties of the purified enzyme were similar to those of the Aerococcus viridans lactate oxidase.
- 1998-05-25
著者
-
Nishiya Yoshiaki
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
-
TODA ATSUSI
Tsuruga Institute of Biotechnology, Toyobo Co. Ltd.
-
Toda Atsusi
Tsuruga Institute Of Biotechnology Toyobo Co. Ltd.
関連論文
- Spontaneous Reactivation of Shiga Toxins in Escherichia coli O157:H7 Cells Caused by Transposon Excision
- Identification of Shiga Toxin-Producing Escherichia coli Possessing Insertionally Inactivated Shiga Toxin Gene
- Gene Cloning, Purification, and Characterization of a Lactate Oxidase from Lactococcus lactis subsp. cremoris IFO3427
- Host-Dependent Activation of IS1203v Excision in Shiga Toxin-Producing Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Identification of an Insertion Sequence, IS1203 Variant, in a Shiga Toxin 2 Gene of Escherichia coli O157:H7
- Gene Cloning and Polymerase Chain Reaction with Proliferating Cell Nuclear Antigen from Thermococcus kodakaraensis KOD1(Biochemistry & Molecular Biology)
- Analysis of a Negative Regulator, SoxR, for the Arthrobacter Sarosine Oxidase Gene
- Characterization of a NADH : Dichloroindophenol Oxidoreductase from Bacillus subtilis
- Cloning and Sequencing of the Sarcosine Oxidase Gene from Arthrobacter sp. TE1826
- Highly Conservative Sequence in the Carboxyl Terminus of Sarcosine Oxidase is Important for Substrate Binding
- Cloning and Nucleotide Sequence of the Urea Amidolyase Gene from Candida utilis
- Primary Structure of the ADEl Gene from Candida utilis