Synthesis of Enzymatically Active Firefly Luciferase in Yeast(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A recombinant plasmid containing a DNA segment complementary to the mRNA of the North American firefly, Photinus pyralis, luciferase was constructed and cloned into Escherichia coli. The cDNA was inserted into a yeast expression vector, AAH5, designed to express active luciferase under the control of the yeast alcohol dehydrogenase promoter. When introduced into the yeast, Saccharomyces cerevisiae, the resultant plasmid directed the synthesis of enzymatically active firefly luciferase, the activity of which was inhibited by antiserum raised against the purified firefly luciferase.
- 社団法人日本農芸化学会の論文
- 1988-05-23
著者
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Nakano Eiichi
Research And Development Division Kikkoman Corporation
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Nakano E
Kikkoman Corp. Chiba Jpn
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TATSUMI Hiroki
Research and Development Division, Kikkoman Corporation
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Tatsumi Hiroki
Research And Development Division Kikkoman Corporation
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Masuda Tsutomu
Research And Development Division Kikkoman Corporation
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