Cloning and Expression of the Creatinase Gene from Flavobacterium sp. U-188 in Escherichia coli(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The gene coding for creatinase (creatine amidinohydrolase, EC 3.5.3.3) was isolated from Flavobacterium sp. U-188. The primary structure of creatinase deduced from the nucleotide sequence showed a protein (molecular weight, 42, 651) composed of 378 amino acids. The creatinase gene was over-expressed in Escherichia coli under the control of the lac promoter and the amount of this enzyme was over 20 % of the soluble protein in the cell.
- 社団法人日本農芸化学会の論文
- 1990-06-23
著者
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Koyama Y
Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer
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Nakano Eiichi
Research And Development Division Kikkoman Corporation
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Nakano Eiichi
Department Of Cardiovascular Surgery Research Institute Of Angiocardiology Kyushu University Faculty
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Koyama Yasuji
Research And Development Division Kikkoman Corporation
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Koyama Yu
Cellular Biochemistry School Of Food And Nutritional Sciences University Of Shizuoka
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Koyama Yu
Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer
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Yamamoto-otake Hideko
Research And Development Division Kikkoman Corporation
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Nakano E
Kikkoman Corp. Chiba
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Suzuki M
National Research Inst. Fisheries Science Ministry Of Agriculture Forestry And Fisheries Tokyo Jpn
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Kitao Satoshi
Research And Development Division Kikkoman Corporation
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SUZUKI Masaru
Noda Institute for Scientific Research
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Kitao Satoshi
Research and Development Division, Kikkoman Corporation
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