Isolation of Lectins by Affinity Chromatography with Porcine Plasma Proteins Immobilized on Agarose(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
To develop a convenient method to isolate lectins, we prepared an affinity gel by coupling plasma proteins with agarose beads under conditions where the pH did not exceed 7.5. The validity of the use of this affinity gel in combination with elution using a hapten saccharide was confirmed by isolation of concanavalin A from Jack bean meal. Successful application of the method was demonstrated by isolation of two novel vegetable lectins from udo (Aralia cordate) and wasabi (Wasabia japonica). The method would be useful to isolate new lectins from various sources including plant and animal tissues.
- 社団法人日本農芸化学会の論文
- 2003-09-23
著者
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長谷 純宏
阪大・理・化学
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Munakata Hiroshi
Department of Urology and Biochemistry, Kinki University, School of Medicine
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長谷 純宏
大阪大学 大学院理学研究科化学専攻
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HASE Sumihiro
Department of Chemistry, Graduate School of Science, Osaka University
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MEGA Tomohiro
Department of Chemistry, Graduate School of Science, Osaka University
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Kajiya Akane
静岡県立大学 食品栄養科学部
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Kajiya Akane
School Of Food And Nutritional Sciences And Center Of Excellence For The 21st Century University Of
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Koyama Y
Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer
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Mega T
Department Of Chemistry Graduate School Of Science Osaka University
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Mega Tomohiro
Department Of Chemistry Graduate School Of Science Osaka University
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Koyama Yu
Cellular Biochemistry School Of Food And Nutritional Sciences University Of Shizuoka
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Koyama Yu
Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer
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KOYAMA Yu
School of Food and Nutritional Sciences and Center of Excellence for the 21st Century, University of
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ISEMURA Mamoru
School of Food and Nutritional Sciences and Center of Excellence for the 21st Century, University of
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MITA Takashi
School of Food and Nutritional Sciences, University of Shizuoka
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KAWAKAMI Toshioki
Sanpo Co., Ltd.
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HONDA Eiko
Life Science Institute, Kinki University
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Honda Eiko
Life Sci. Res. Inst. Kinki Univ.
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Honda E
Life Science Institute Kinki University
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Mita T
School Of Food And Nutritional Sciences University Of Shizuoka
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Mita Takashi
Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer
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Munakata Hiroshi
Department Of 2nd Biochemistry Kinki University School Of Medicine
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Kawakami Toshioki
Sanpo Co. Ltd.
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Koyama Yu
School Of Food And Nutritional Sciences And Center Of Excellence For The 21st Century University Of
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Hase Sumihiro
Department Of Chemistry Graduate School Of Science Osaka University
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Hase Sumihiro
Department Of Chemisty Graduate School Ofscience Osaka University
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Mita Takashi
Department Of Chemistry Faculty Of Science And Technology Keio University
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MUTO Haruhiko
Shizuoka Prefecture Forestry and Forest Products Research Institute
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伊勢村 護
Cellular Biochemistry School Of Food And Nutritional Sciences University Of Shizuoka
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Isemura Mamoru
Laboratory Of Cellular Biochemistry Graduate School Of Nutritional And Environmental Sciences Univer
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Mita T
Tohoku Univ.
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