Characterization of an Apoptosis-Inducing Factor in Habu Snake Venom as a Glycyrrhizin (GL)-binding Protein Potently Inhibited by GL in VItro

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概要

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• By means of successive heparin-affinity and glycyrrhizin (GL)-affinity column chromatographies (HPLC), a 55 kDa GL-binding protein (gp55) was purified to apparent homogeneity from the Superdex P-I fraction of Habu snake venom. This gp55 was identified as an apoxin I-like protein, because (i) its 20 N-terminal amino acid residues (AHDRNPLEEYFRETDYEEFL) are 95% identical with the corresponding sequence of apoxin I (apoptosis-inducing factor, approx. 55 kDa) in the venom of the western diamondback rattlesnake; and (ii) L-amino acid oxidase (LAO) activity of gp55 is detected when incubated with L-leucine, but not with D-leucine. GL inhibited the LAO activity of gp55 in a dose-dependent manner, but had no effect on the activity of a 65 kDa LAO also purified from Habu snake venom. In addition, GL reduced the ability of gp55 to induce the hemolysis of sheep red blood cells. These results suggest that GL is a potent inhibitor of apoxin I-like proteins in harmful snake venoms.

• 公益社団法人日本薬学会の論文
• 1998-09-15

著者

• OHTSUKI Kenzo

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• NAGATA NOBUYUKI

  Research Laboratory, Minophagen Co.

• Munakata Hiroshi

  Department of Urology and Biochemistry, Kinki University, School of Medicine

• Nagata N

  Minophagen Pharmaceutical Co. Ltd. Kanagawa Jpn

• Nagata Nobuyuki

  Research Laboratories Of Minophagen Pharmaceutical Co. Ltd.

• Ito J

  Laboratory Of Biochemistry Sagami Women's University

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• ABE Yoshinori

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• SHIMOYAMA Yoshihito

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• ITO Junko

  Laboratory of Biochemistry, Sagami Women's University

• 大久保 研之

  Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University

• MUNAKATA Haruka

  Hokkaido College of Pharmacy

• Munakata Hiroshi

  Department Of 2nd Biochemistry Kinki University School Of Medicine

• Munakata Hiroshi

  Dept. Of Biochemistry Kinki Univ. School Of Med.

• Nagata Naoki

  Institute Of Applied Biochemistry University Of Tsukuba

• MUTO Haruhiko

  Shizuoka Prefecture Forestry and Forest Products Research Institute

• Abe Yoshinori

  Laboratory Of Genetical Biochemistry Kitasato University School Of Allied Health Sciences

• Munakata Hidekazu

  Department Of Biochemistry Kinki University School Of Medicine

• Shimoyama Y

  Kitasato Univ. Sagamihara Jpn

• Yanahira Shuichi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Ito Junko

  Laboratory Of Biochemistry Sagami Women's University Kitasato University

• ITO JUNKO

  Faculty of Pharmacy, Meijo University

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