Inhibitory Effect of Glycyrrhizin on the Phosphorylation and DNA-Binding Abilities of High Mobility Group Proteins 1 and 2 in Vitro
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概要
- 論文の詳細を見る
The physiological correlation between glycyrrhizin(GL)and high mobility group proteins 1 and 2(HMG1/2)and the inhibitory effect of GL on their phosphorylation by three protein kinases(CK-I, CK-II and PKC)were investigated biochemically in vitro. It was found that GL binds directly to HMG1/2, because(i)HMG1/2 have a high affinity with a GL-affinity column ; and(ii)GL induces the conformational changes in HMG1/2 . Both purified HMG1/2 functioned as phosphate acceptors for these two protein kinases(CK-I and PKC), but not phosphorylated by CK-II. Phosphorylation of HMG1/2 by two protein kinases(CK-I and PKC)was completely inhibited by a glycyrrhetinic acid derivative(οGA)at one-tenth the concentration of GL. Also, the DNA-binding abilities of HMG1/2 were reduced by GL in a dose-dependent manner. These results show that the binding of GL to HMG1/2 results in the inhibition of their physiological activities(DNA-binding ability and phosphorylation by PKC or CK-I)in vitro. The GL-induced inhibition of the physiological activities of HMG1/2 may be involved in the anti-inflammatory effect of GL in vivo.
- 公益社団法人日本薬学会の論文
- 2001-08-01
著者
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OHTSUKI Kenzo
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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Sakamoto Ryoko
Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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Sakamoto Ryoko
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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OKANO Maiko
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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Okano Maiko
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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TAKENA Hiroko
Laboratory of Genetical Biochemistry, Graduate School of Medical Sciences, Kitasato University
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Takena Hiroko
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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