Biochemical Characterization of Selective Inhibitors for a Glycyrrhizin-binding Lipoxygenase from Soybeans

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概要

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• By GL-affinity column chromatography (HPLC), a GL-binding lipoxygenase (gbLOX) was selectively purified from a partially purified soybean LOX-1 fraction. gbLOX was identified as LOX-3 since the partial N-terminal amino acid sequences of at least four fragments generated from the purified gbLOX (p96 and p94) digested with trypsin were identical to the corresponding sequences of soybean LOX-3. Thb inhibitory effects of various flavonoids and their modified derivatives, epigallocatechin gallate (EGCG) and a derivativc (oGA) of glycyrrhetinic acid on the activities of two LOXs [gbLOX and ngLOX (non-GL-binding LOX)] were examined in vitro. It was found that (i) four compounds [quercetin, EGCG, a derivative (2',4'-DDC) of 3,4-dihydroxychalcone, and oGA] inhibit gbLOX activity in a dose-dependent manner, but do not affect ngLOX activity; and (ii) quercetin as well as EGCG effectively prevents phosphorylation of gbLOX by casein kinase II (CK-II) in vitro. The results provided here suggest that the potent gbLOX inhibitors function as suppressors for the LOX-3-catalyzed metabolic pathways in plant cells.

• 社団法人日本農芸化学会の論文
• 1997-03-23

著者

• OHTSUKI Kenzo

  Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa

• NAGATA NOBUYUKI

  Research Laboratory, Minophagen Co.

• SATOH Toshio

  Faculty of Pharmaceutical Sciences, Tokushima Bunri University

• Nagata N

  Minophagen Pharmaceutical Co. Ltd. Kanagawa Jpn

• Nagata Nobuyuki

  Research Laboratories Of Minophagen Pharmaceutical Co. Ltd.

• Ohtsuki K

  Kitasato Univ. Sagamihara Jpn

• Ohtsuki Kenzo

  Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato

• TOMIMORI Tsuyoshi

  School of Pharmacy, Hokuriku University

• SHIMOYAMA Yoshihito

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• FURUYA Teisuke

  Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health Sciences

• 大久保 研之

  Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University

• Nagata Naoki

  Institute Of Applied Biochemistry University Of Tsukuba

• Ohtsuki Kozo

  Department Of Food Sciences And Nutritional Health Kyoto Prefectural University

• Furuya Teisuke

  Laboratory Of Genetical Biochemistry Kitasato University School Of Allied Health Sciences

• Shimoyama Y

  Kitasato Univ. Sagamihara Jpn

• Yanahira Shuichi

  Technology And Research Institute Snow Brand Milk Products Co. Ltd.

• Satoh Toshio

  Faculty Of Pharmaceutical Sciences Tokushima Bunri University

• Tomimori Tsuyoshi

  School Of Pharmacy Hokuriku University

• Satoh Toshio

  Faculty Of Pharmaceutical Science Tokushima Bunri University

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