Characterization of Bovine Angiogenin-1 and Lactogenin-Like Protein as Glycyrrhizin-Binding Proteins and Their in Vitro Phosphorylation by C-kinase
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概要
- 論文の詳細を見る
Angiogenin-1 (p15, an angiogenesis inducer with RNase activity) and lactogenin-like protein (p17) isolated from partially purified bovine lactoferrin (bLF) preparations were characterized as glycyrrhizin (GL)-binding proteins (gbPs). As expected, bLF-affinity column chromatography confirmed these two gbPs to be bLF-binding proteins. These two purified gbPs exhibited RNase activities when incubated with poly (C) as a substrate. Both GL and glycyrrhetinic acid (GA) at 100μM significantly inhibited RNase activities of these two gbPs, both of which functioned as phosphate acceptors of C-kinase in vitro. Phosphorylation of p15 and p17 by C-kinase was inhibited by GA in a dose-dependent manner with the 50% inhibition dose (ID_50) of approx. 10μM, whereas GL required a relatively high dose (300μM) to inhibit significantly it. A GA derivative (oGA, ID_50=approx.0.3μM) was found to be a potent inhibitor of the C-kinase-mediated phosphorylation of these two gbPs in vitro. In addition, a possible physiological significance of C-kinase on the physiological interaction between bLF and two bLF-binding proteins (p15 and p17) is noted.
- 公益社団法人日本薬学会の論文
- 2001-05-01
著者
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Tanaka Katsunao
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Tanaka Katsunao
大鵬薬品工業
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Tanigawa Kiyoshi
大鵬薬品工業
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Tanigawa Kiyoshi
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Ohtsuki K
Kitasato Univ. Sagamihara Jpn
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Ohtsuki Kenzo
Genetical Biochem. Kitasato Univ.
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Fujihara M
Kitasato Univ. Sagamihara Jpn
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Fujihara Michio
Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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大久保 研之
Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University
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Sakamoto R
Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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Ohtsuki K
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Tanigawa Kozo
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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TANIGAWA KOZO
Laboratory of Chinical Chemistry, School of Allied Health Sciences, Kitasato University
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FUJIHARA Michio
Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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SAKAMOTO Ryoko
Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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YANAHIRA Shuichi
Technology and Research Institute, Snow Brand Milk Products Co., Ltd.
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Shimoyama Y
Kitasato Univ. Sagamihara Jpn
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Yanahira Shuichi
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Tanigawa Kozo
Laboratory Of Chinical Chemistry School Of Allied Health Sciences Kitasato University
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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YANAHIRA Shuichi
Technical Research Institute, Snow Brand Milk Products Co. Ltd.
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