Expression and Characterization of Human Rheumatoid Factor Single-Chain FV
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概要
- 論文の詳細を見る
The variable region of heavy chain (V_H) of human rheumatoid factor (hRF) IgH was connected with the variable region of light chain (V_L) with the peptide-linker (GGGSGGGSGGGS) by genetic engineering method and the single-chain FV (scFv) was expressed in E. coli. On design, scFv and scFv (tag) were planned; the latter had a detection marker at the carboxyl-terminal. These scFvs were expressed as inclusion bodies in E. coli, purified in the presence of 8 M urea by gel filtration and renatured to the active form in vitro. As a control, the Fv, non-covalently associated V_H and V_L fragements, was also constructed. The 3 derivatives showed almost the same binding activity to rabbit-IgG to which hRF is cross-reactive. ScFv (tag) was the most stable against urea among the 3 derivatives.
- 社団法人日本薬学会の論文
- 2000-08-01
著者
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Tanaka Katsunao
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Tanaka Katsunao
大鵬薬品工業
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Tanigawa Kiyoshi
大鵬薬品工業
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Tanigawa Kiyoshi
Graduate School Of Pharmaceutical Sciences Kyushu University
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Tanigawa Kiyoshi
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Ikenaga Takeshi
Graduate school of Information, Production and Systems, Waseda University
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Ikenaga T
Graduate School Of Pharmaceutical Sciences Kyushu University
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Ikenaga Takeshi
Graduate School Of Information Production And System Waseda University
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Ueda T
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Ueda T
Graduate School Of Pharmaceutical Sciences Kyushu University
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IMOTO Taiji
Faculty of Biotechnology and Life Science, Sojo University
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UEDA Tadashi
Graduate School of Pharmaceutical Sciences, Kyushu University
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IMOTO Taiji
Graduate School of Pharmaceutical Sciences, Kyushu University
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HASHIMOTO Yoshio
Graduate School of Pharmaceutical Sciences, Kyushu University
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EZAKI Ichiko
Department of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University
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Ezaki Ichiko
Department Of Clinical Immunology Medical Institute Of Bioregulation Kyushu University
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Imoto T
Faculty Of Biotechnology And Life Science Sojo University
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Imoto Taiji
Graduate School Of Pharmaceutical Sciences
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Imoto Taiji
Graduate School Of Pharmaceutical Science Kyushu University
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Ueda Takuya
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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上田 卓也
東京大学大学院新領域創成科学研究科メディカルゲノム専攻
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Ueda Tadashi
Graduate School Of Pharmaceutical Sciences Kyushu Univ.
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Ueda Tadashi
Graduate School Of Pharmaceutical Sciences
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Imoto Taiji
九州大学 薬学研究院
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Hashimoto Y
Graduate School Of Pharmaceutical Sciences Kyushu University
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Hashimoto Yuichi
Institute Of Molecular & Cellular Biosciences University Of Tokyo
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Tanigawa Kozo
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Hashimoto Yoshio
Graduate School Of Pharmaceutical Sciences Kyushu University
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IKENAGA Takeshi
Graduate School of Fundamental Science and Engineering, Waseda University
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