MORPHOLOGICAL CHANGE AND INACTIVATION OF HUMAN LUNG MYOFIBROBLASTS CULTURED IN CONDITIONED MEDIUM OF HUMAN LUNG CARCINOMA

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Relationship between lung cancer and lung fibrosis has not been well understood yet. For example, the precancerous lesion of the pulmonary adenocarcinoma does not result from the nest of the fibrosis. In the present study, we investigated mainly the expression of α-smooth muscle actin (SMA), an important marker of activation, of the MRC-5 human lung myofibroblasts treated with conditioned medium of the A549 human lung carcinoma cultured for 4 days. The shape of the cells treated with the conditioned medium under M.W. 10,000 changed remarkably. The number of the cells treated with the conditioned medium for 3 days did not increased, however, apoptosis was not observed by flow cytometric analysis. While only a single cell population was identified in the cells treated with DMEM, two cell populations were observed in the cells treated with the conditioned medium by flow cytometric profiles analyzed on FSC and SSC. Western blot analysis showed the effect of the conditioned medium on α-SMA expression. The intensity of α-SMA band decreased in the cells treated with the conditioned medium for 7 days. However, the expression of a-SMA in the cells treated with DMEM was almost the same level on each day. Immunohistochemical staining of the cells showed the same results as the western blot analysis. Therefore, the effect of some factors in the conditioned medium of lung carcinoma found here may represent an important mechanism for prevention of lung fibrosis.
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MORPHOLOGICAL CHANGE AND INACTIVATION OF HUMAN LUNG MYOFIBROBLASTS CULTURED IN CONDITIONED MEDIUM OF HUMAN LUNG CARCINOMA

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