Transglucosylation Catalyzed by Sucrose Phosphorylase from Leuconostoc mesenteroides and Production of Glucosyl-xylitol
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概要
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The synthesis of glucooligosaccharides from α-D-glucose-1-phosphate by transglucosylation with sucrose phosphorylase from Leuconostoc mesenteroides was studied using the purified enzyme and high performance liquid chromatography. The enzyme had a rather broad acceptor specificity and transferred glucosyl residues to various acceptors such as sugars and sugar alcohols. Especially. 5-carbon sugar alcohols (pentitols), D- and L-arabitol were acceptors equal to D-fructose, which was known as a good acceptor. The transfer product of xylitol formed by the enzyme was investigated. The structure of the product was found to be 4-O-α-D-glucopyranosyl-xylitol (G-X) by acid hydrolysis and ^<13>C-nuclear magnetic resonance analysis. G-X is a probable candidate for a preventive for dental caries because it reduced the synthesis of water-insoluble glucan by Streptococcus mutans and kept a neutral pH in the cell suspension.
- 社団法人日本農芸化学会の論文
- 1992-12-23
著者
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Kitao Satoshi
Research And Development Division Kikkoman Corporation
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Sekine Hiroshi
Research And Development Division Kikkoman Corporation
関連論文
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- Enzymatic Syntheses of Two Stable (-)-Epigallocatechin Gallate-glucosides by Sucrose Phosphorylase
- The Syntheses of Catechin-glucosides by Transglycosylation with Leuconostoc mesenteroides Sucrose Phosphorylase
- Purification and Some Properties of Sucrose Phosphorylase from Leuconostoc mesenteroides(Biological Chemistry)
- Cloning of the Sucrose Phosphorylase Gene from Leuconostoc mesenteroides and Its Overexpression Using a 'Sleeper' Bacteriophage Vector
- Syntheses of Two Kojic Acid Glucosides with Sucrose Phosphorylase from Leuconostoc mesenteroides
- Formation of Kojibiose and Nigerose by Sucrose Phosphorylase
- Cloning and Sequence Analysis of the Gene for Glucodextranase from Arthrobacter globiformis T-3044 and Expression in Escherichia coli Cells
- α-D-Glucosyl Transfer to Phenolic Compounds by Sucrose Phosphorylase from Leuconostoc mesenteroides and Production of α-Arbutin
- Transglucosylation Catalyzed by Sucrose Phosphorylase from Leuconostoc mesenteroides and Production of Glucosyl-xylitol