Purification and Characterization of a Thermostable Pyruvate Kinase from the Actinomycete Microbispora thermodiastatica
スポンサーリンク
概要
- 論文の詳細を見る
The pyruvate kinase of Microbispora thermodiastatica was purified to homogeneity and some properties of the enzyme were characterized. The molecular weight of the enzyme by gel filtration is 277,000. The subunit molecular weight is 55,000 by SDS-polyacrylamide gel electrophoresis, and only one N-terminal amino acid sequence was obtained. It had d pH optimum around pH 4.5 to 7.0 and was stable over the range of pH 4.0-8.0. The enzyme is thermostable and no activity was lost after heat treatment at 55℃ for 60 min. AMP activated this enzyme and the saturation curve of the enzyme for PEP changed from sigmoidal type to hyperbolic type in the presence of AMP.
- 社団法人日本農芸化学会の論文
- 1997-01-23
著者
-
Murakami Seiji
Research And Development Division Kikkoman Corporation
-
Arai Ayumi
Research and Development Division, Kikkoman Corporation
-
Nakajima Moto-o
Research and Development Division, Kikkoman Corporation
-
Arai Ayumi
Research And Development Division Kikkoman Corporation
-
Nakajima Moto-o
Research & Development Division Of Kikkoman Corpration
-
Nakajima Moto-o
Research And Development Division Kikkoman Corporation
-
Nakajima Motoo
Research and Development Division, Kikkoman Corporation
関連論文
- Bioluminescent Enzyme Immunoassay for Mouse Interleukin-6 Using Acetate Kinase
- Enhancement of the Thermostability of the Alkaline Protease from Aspergillus oryzae by Introduction of a Disulfide Bond
- Autoproteolytic Processing and Processing-dependent Secretion of Aspergillus oryzae Alkaline Protease in Yeast(Biological Chemistry)
- Isolation and Characterization of the Alkaline Protease Gene of Aspergillus oryzae(Biological Chemistry)
- Secretion of Aspergillus oryzae Alkaline Protease in an Osmophilic Yeast, Zygosaccharomyces rouxii(Biological Chemistry)
- Cloning anc Sequencing of the Alkaline Protease cDNA from Aspergillus oryzae(Microbilolgy & Fermentation Industry)
- Purification and Characterization of a Thermostable Pyruvate Kinase from the Actinomycete Microbispora thermodiastatica
- Mutant Luciferase Enzymes from Fireflies with Increased Resistance to Benzalkonium Chloride(Biochemistry & Molecular Biology)
- Assay of Methylguanidine As a Hydroxyl Radical Marker in Serum of Patients on Hemodialysis
- Purification and Properties of Guanidinoacetate Kinase from a Polychaete, Perinereis sp.(Biological Chemistry)