Base Specificity and Primary Structure of Poly U-preferential Ribonuclease from Chicken Liver
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概要
- 論文の詳細を見る
The primary structure and base specificity of chicken liver RNase CL_1 which has been reported by Miura et al. [Chem. Pharm. Bull., 32, 4053-4060 (1984)] as poly U-preferential RNase, were extensively studied. The sequence study of this enzyme and comparison of the amino acid sequence of the enzyme with homologous RNases from oyster and Drosophila melanogaster suggested that RNase CL_1 consists of three peptides with 17, 19, and 163 amino acid residues. The amino acid sequence of these three peptides were identified. The two small peptides are joined to the large peptide by disulfide bridges. The amino acid sequence of RNase CL_1 had 62 (31.2%) and 63 residues (31.6%) identical with oyster RNase and D. melanogaster RNase, respectively, and belongs to the RNase T_2 family RNase. Reassessment of the base specificity of RNase CL_1 found that it is guanylic acid, then uridylic acid-preferential, and not poly U preferential.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
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Uchida Takashi
Department of Urban Engineering, Osaka City University
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Watanabe Hideaki
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi Kazuko
Department of Microbiology, Hoshi College of Pharmacy
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Ohgi K
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi Kazuko
Department Of Microbiology Hoshi College Of Pharmacy
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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IWAMA Masanori
Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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Watanabe H
Univ. Tokyo Tokyo Jpn
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Watanabe Hideaki
Department Of Microbiology Hoshi College Of Pharmacy
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Sanda A
Department Of Hygienic Technology College Of Environmental Health Azabu University
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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Uchida T
Hiroshima Univ. Graduate School Of Biomedical Sci. Hiroshima Jpn
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HAYANO Kumi
Department of Microbiology, Hoshi College of Pharmacy
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SANDA Akihiro
Faculty of Public Health, Azabu University
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Watanabe Hideaki
Department Of Dermatology Showa University School Of Medicine
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Uchida Takashi
Department Of Electronic Engineering National Defense Academy
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Watanabe Hideaki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Ohgi Kazuko
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Ohgi K
Department Of Microbiology Hoshi College Of Pharmacy
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Watanabe Hideaki
Department of Applied Biological Chemistry, The University of Tokyo
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Uchida Takashi
Department of Electrical and Electronic Engineering, National Defense Academy
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Uchida Takashi
Department of Clinical Pharmaceutical Sciences, School of Pharmaceutical Sciences, Kumamoto University
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WATANABE HIDEAKI
Department of Anesthesiology, Kurume University School of Medicine
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