Loss of a GPI-Anchored Membrane Protein Aah3p Causes a Defect in Vacuolar Protein Sorting in Schizosaccharomyces pombe
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概要
- 論文の詳細を見る
Schizosaccharomyces pombe has four α-amylase homologs (Aah1p-Aah4p) with a glycosylphosphatidylinositol (GPI) modification site at the C-terminal end. Disruption mutants of aah genes were tested for mislocalization of vacuolar carboxypeptidase Y (CPY), and aah3Δ was found to secrete CPY. The conversion rate from pro- to mature CPY was greatly impaired in aah3Δ, and fluorescence microscopy inidicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. These results indicate that aah3Δ had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting.
- 社団法人 日本農芸化学会の論文
著者
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Tanaka Naotaka
Department of Life Science, Kagawa University
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TAKEGAWA Kaoru
Department of Life Science, Faculty of Agriculture, Kagawa University
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Iwaki Tomoko
Dep. Of Applied Bioresource Sci. Fac. Of Agriculture Ehime Univ.
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Iwaki Tomoko
Department Of Biological Resources Faculty Of Agriculture Ehime University
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MORITA Tomotake
Department of Life Sciences, Faculty of Agriculture, Kagawa University
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GIGA-HAMA Yuko
Research Center, Asahi Glass Co., Ltd.
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