Biopanning of Antibody-Phage Clones Using Immunoplates Coated with Gel Slices of Electrophoresis : Immunogel-Biopanning(Analytical Biochemistry)

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概要

• 論文の詳細を見る

• Biopanning of a phage library using a Western blotting membrane is difficult because of high background binding. We propose a reliable biopanning method, namely, immunogel-biopanning, which is performed using immunoplates coated with a molecular species fractionated from a crude sample by native polyacrylamide gel electrophoresis (PAGE). The efficacy of this method was determined in model experiments using a human interleukin-18 (IL-18)-specific single chain Fv (scFv) phage clone.

• 社団法人日本薬学会の論文
• 2007-08-01

著者

• Yoshihara Tomoki

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Uchida Shunsuke

  Kagoshima Univ. Kagoshima Jpn

• Ito Yuji

  Kagoshima Univ. Kagoshima

• HASHIGUCHI Shuhei

  Department of Bioengineering, Faculty of Engineering, Kagoshima University

• ITO Yuji

  Department of Bioengineering, Faculty of Engineering, Kagoshima University

• SUGIMURA Kazuhisa

  Department of Bioengineering, Faculty of Engineering, Kagoshima University

• HAMASAKI Takayuki

  Department of Bioengineering, Faculty of Engineering, Kagoshima University

• UCHIDA Shunsuke

  Department of Bioengineering, Faculty of Engineering, Kagoshima University

• Hashiguchi S

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Hashiguchi Shuhei

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Hashiguchi Shuhei

  Department Of Molecular Biology Faculty Of Engineering Kagoshima University

• Uchida Shunsuke

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Ito Yuji

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Sugimura Kazuhisa

  Department Of Molecular Biology Faculty Of Engineering Kagoshima University

• Sugimura Kazuhisa

  Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Kagoshima University

• ITO Yuji

  National Institute of Agrobiological Sciences

• Hashimoto Tadafumi

  Department Of Bioengineering Faculty Of Engineering Kagoshima University

• Sugimura Kazuhisa

  Collaborative Development Of Innovation Seeds Japan Science And Technology Corporation

• Ito Y

  Inst. Physical And Chemical Res. (riken) Saitama Jpn

• Hashiguchi Shuhei

  Department Of Chemistry Biotechnology And Chemical Engineering Graduate School Of Science And Engineering Kagoshima University

• Sugimura Kazuhisa

  Department Of Chemistry Biotechnology And Chemical Engineering Graduate School Of Science And Engineering Kagoshima University

• Ito Yuji

  Department of Appleed Chemiaity and Bialownology,Facutory of Enginiaring,niigata Institute of Technology

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