Purification of Phosphoproteins NrEP1 to 6, Markers for the Beginning of Pollen Embryogenesis in Nicotiana rustica, and Identification of Their N-terminal Sequences
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概要
- 論文の詳細を見る
In pollen culture methods developed for Nicotiana rustica and N. tabacum, it is possible to induce embryogenic dedifferentiation, i.e., transformation process from immature pollen to embryogenic cells. As biochemical markers for the dedifferentiation of pollen attention has been focused on several phosphoproteins which characteristically appeared in the two-dimensional gel electrophoretogram of total protein from pollen fed with 32Pi in both species. Here, the six phosphoproteins of N. rustica, NrEP1 to 6, were partially purified and their N-terminal amino acid sequences were identified. The sequences were similar to each other and also to those of the phosphoproteins (NtEPs) previously identified in N. tabacum. This suggests that a protein family possessing a common structure plays an important role in the induction process of pollen embryogenesis in Nicotiana.
- 日本植物細胞分子生物学会の論文
- 2002-12-01
著者
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FUKUI HIROSHI
Department of Biopharmaceutics, Kyoto Pharmaceutical University
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Yamaji Naoki
Department Of Life Sciences Faculty Of Agriculture Kagawa University
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HATTORI Shoji
Soda Aromatic Co., Ltd., Analysis Research Department
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KYO Masaharu
Department of Life Sciences, Faculty of Agriculture, Kagawa University
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Kyo Masaharu
Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Kyo Masaharu
Department Of Bioresource Science Faculty Of Agriculture Kagawa University
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Hattori Shoji
Soda Aromatic Co. Ltd.
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Fukui Hiroshi
Department Of Applied Biological Science Faculty Of Agriculture Kagawa University
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Fukui Hiroshi
Department Of Life Sciences Faculty Of Agriculture Kagawa University
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