Effects of EDTA and Acidified Medium on the Dedifferentiation of Immature Pollen in a Tobacco Pollen Culture

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概要

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• The addition of ethylenediaminetetraacetic acid (EDTA) to the medium stimulated the dedifferentiation from immature pollen grains to embryogenic cells in the tobacco pollen culture system developed by Kyo and Harada (1986). The stimulative effect of EDTA was mainly due to the decrease in the pH of the medium and was very marked in the pollen at the late-bicellular stage (stage IV), which did not become embryogenic under the original culture condition.

• 日本植物生理学会の論文

著者

• Kyo Masaharu

  Department Of Bioresource Science Faculty Of Agriculture Kagawa University

関連論文

• Cloning of cDNA Encoding NtEPc, a Marker Protein for the Embryogenic Dedifferentiation of Immature Tobacco Pollen Grains Cultured in Vitro
• Purification of Phosphoproteins NrEP1 to 6, Markers for the Beginning of Pollen Embryogenesis in Nicotiana rustica, and Identification of Their N-terminal Sequences
• Effects of EDTA and Acidified Medium on the Dedifferentiation of Immature Pollen in a Tobacco Pollen Culture

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