Glutamine Production by Coupling with Energy Transfer: Employing Yeast Cell-Free Extracts and Gluconobacter Glutamine Synthetase
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概要
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Glutamine production was investigated by coupling of glutamine synthetase from Gluconobacter suboxydans with a sugar fermentation system of bakers yeast (energy generating system). Under the optimum condition, 22 mM glutamine was formed in 3 hr, and the yield was 92 % based on the substrate glutamate. The first step of the process was the accumulation of fructose 1, 6-diphosphate (FDP) as a reservoir of fermentation energy, in the presence of a high concentration of inorganic phosphate ; and the second step was accomplished by coupling the degradation of FDP with glutamine synthetase reaction through an ADP-ATP system. The effects of enzyme concentration, additives in the reaction mixture and others on glutamine formation were investigated, and the importance of three factors was pointed out : (a) the ratio of activity of energy generating system to utilizing system, (b) contaminated enzyme(s) in the energy utilizing system and (c) the enzymatic properties of the energy utilizing system.
- 社団法人 日本農芸化学会の論文
著者
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Tochikura Tatsurokuro
Department Of Agricultural Chemistry Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Matsumoto Hiroko
Department Of Astronomy School Of Science Kyoto University
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YANO Toshihiro
Department of Food Science and Technology, Kyoto University
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