Hydrolysis of α-D-Galactosyl Oligosaccharides in Soymilk by α-D-Galactosidase of Bifidobacterium breve 203(Microbiology & Fermentation Industry)

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概要

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• Bifidobacterium breve 203 grew well on soymilk, reaching a cell number of 2×10^9 cells/ml on 24hr cultivation, at which time the medium had solidified and its pH had decreased to 4.0. Stachyose and raffinose in the medium were assimilated in preference to sucrose. A crude extract of B.breve 203 hydrolyzed the oligosaccharides in soymilk almost completely into their constituent monosaccharides. The α-D-galactosidase responsible for the first reaction in the degradation of the α-D-galactosyl oligosaccharides was isolated from a crude extract of the organism (500-fold purification, 2% yield) by means of ammonium sulfate-fractionation and column chromatographies on DEAE-cellulose, DEAE-cellulofine, Sepharose 6B, hydroxylapatite, Sephacryl S-300 and Phenyl-Sepharose 6B. The enzyme was a homo-octamer with a molecular weight of about 330,000, and its isoelectric point was 3.7. It reacted optimally at pH 5.5 and hydrolyzed stachyose (Km 8.6 mM), raffinose (km 4.4 mM) and melibiose (Km 3.0 mM), with 50〜60% of its reactivity toward p-soymilk and guar gum was also investigated.

• 社団法人日本農芸化学会の論文
• 1987-02-23

著者

• Sakai Kenji

  Department of Plant Resources, Kyushu University

• TACHIKI TAKASHI

  Department of Food Science and Technology, Kyoto University

• KUMAGAI HIDEHIKO

  Department of Food Science and Technology, Kyoto University

• TOCHIKURA TATSUROKURO

  Department of Food Science and Technology, Kyoto University

• Kumagai Hidehiko

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Kumagai Hidehiko

  Faculty Of Agriculture Kyoto University

• Tochikura Tatsurokuro

  Department Of Agricultural Chemistry Kyoto University

• Tachiki Takashi

  Department Of Chemistry Ritsumeikan University

• Sakai Kenji

  Department Of Environmental Chemistry And Engineering Faculty Of Engineering Oita University

• Kumagai H

  Department Of Agricultural Chemistry The University Of Tokyo

• Tachiki Takashi

  Faculty Of Home Economics Kobe Women's University

• Tachiki Tkashi

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• Tachiki Takashi

  Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit

• Sakai Kenji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

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