Purification and Characterization of Chitinase A of Streptomyces cyaneus SP-27 : An Enzyme Participates in Protoplast Formation from Schizophyllum commune Mycelia
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概要
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A culture filtrate of Bacillus circulans KA-304 grown on a cell-wall preparation of Schizophyllum commune has an activity to form protoplasts from S. commune mycelia. α-1,3-Glucanase and chitinase I, which were isolated from the filtrate, did not form the protoplast by itself while a mixture of them showed protoplast-forming activity.Streptomyces cyaneus SP-27 was isolated based on the productivity of chitinase. The culture filtrate of S. cyaneus SP-27 did not form S. commune protoplasts, but addition of it to α-1,3-glucanase of B. circulans KA-304 brought about protoplast-forming activity. Chitinase A isolated from the S. cyaneus SP-27 culture filtrate was more effective than chitinase I of B. circulans KA-304 for the protoplast formation in combination with α-1,3-glucanase. The N-terminal amino acid sequence of chitinase A (MW 29,000) has a sequential similarity to those of several Streptomycete family 19 chitinases. Chitinase A adsorbed to chitinous substrate and inhibited the growth of Trichoderma reesei mycelia. Anomer analysis of the reaction products also suggested that the enzyme is a family 19 chitinase.
- 社団法人 日本農芸化学会の論文
- 2008-01-23
著者
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TACHIKI TAKASHI
Department of Food Science and Technology, Kyoto University
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YANO Shigekazu
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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HONDA Arata
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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WAKAYAMA Mamoru
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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Wakayama Mamoru
Dep. Of Biotechnology Fac. Of Life Sciences Ritsumeikan Univ.
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Wakayama Mamoru
Department Of Bioscience And Biotechnology Faculty Of Science And Engineering Ritsumeikan University
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Rattanakit Nopakarn
Department of Chemistry, Faculty of Science, Chiang Mai University
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Tachiki T
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Tachiki Takashi
Department Of Bioscience & Technology Faculty Of Science & Engineering Ritsumeikan Universit
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Yano Shigekazu
Department Of Biotechnology College Of Life Sciences Ritsumeikan University
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Rattanakit Nopakarn
Department Of Chemistry Faculty Of Science Chiang Mai University
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NODA Yuta
Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University
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PLIKOMOL Abhinya
Department of Biology, Faculty of Science, Chiang Mai University
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Yano Shuntaro
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Honda Arata
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Wakayama Mamoru
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Noda Yuta
Department Of Biotechnology Faculty Of Life Sciences Ritsumeikan University
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Plikomol Abhinya
Department Of Biology Faculty Of Science Chiang Mai University
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YANO Shigekazu
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University
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Wakayama Mamoru
Department of Applied Chemistry, Faculty of Engineering, Oita University
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