Development of a Novel Functional High-Throughput Screening System for Pathogen Effectors in the Yeast Saccharomyces cerevisiae
スポンサーリンク
概要
- 論文の詳細を見る
Our understanding of the molecular mechanisms of bacterial pathogenesis has been improved especially by the discovery of host cell contact-dependent secretion systems such as the type-III secretion system (T3SS) found in numerous pathogens. Although the identification of pathogen effectors translocated into host cells through T3SS is essential to the understanding of pathogenesis, their general sequence uniqueness confound attempts to identify such proteins by sequence homology. Here we report the development of a functional high-throughput screening system for pathogen effectors in yeast that consists of a GatewayTM-compatible Tet-Off inducible expression vector and a yeast strain expressing a reporter, facilitating identification of the effectors affecting host vesicular trafficking pathways. We evaluated this system and optimized the screening condition using several known pathogen effectors. We found this system useful in functional characterization of pathogen effector and it can be adapted to functional high-throughput screening as well.
- 社団法人 日本農芸化学会の論文
- 2009-10-23
著者
-
AKADA Rinji
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine
-
Akada Rinji
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
-
TABUCHI Mitsuaki
Department of Bioresource Science, Faculty of Agriculture, Kagawa University
-
KAWAI Yasuhiro
Department of Pediatrics, Kawasaki Medical School
-
OUCHI Kazunobu
Department of Pediatrics, Kawasaki Medical School
-
KISHI Fumio
Department of Microbiology and Immunology, Kagoshima University Graduate School of Medical and Denta
-
Miyashita Naoyuki
Department Of Microbiology Kawasaki Medical School
-
Ouchi Kazunobu
Dep. Of Pediatrics Kawasaki Medical School
-
Ouchi Kazunobu
Department Of Paediatrics Saiseikai Shimonoseki General Hospital
-
Nishie Fujita
Department Of Molecular Genetics Kawasaki Medical School
-
IZUMI Terumi
Department of Molecular Genetics, Kawasaki Medical School
-
YANATORI Izumi
Department of Molecular Genetics, Kawasaki Medical School
-
Miyashita Naoyuki
Department Of Respiratory Diseases Kawasaki Medical School
-
Akada Rinji
Department Of Applied Molecular Bioscience Division Of Engineering Yamaguchi University Graduate Sch
-
Kawai Yasuhiro
Department Of Pediatrics Kawasaki Medical School
-
Kishi Fumio
Department Of Molecular Genetics Kawasaki Medical School
-
Miyashita Naoyuki
Department Of Respiratory Medicine Kawasaki Medical School
-
Tabuchi Mitsuaki
Department Of Molecular Genetics Kawasaki Medical School
-
Izumi Terumi
Department Of Molecular Genetics Kawasaki Medical School
-
Yanatori Izumi
Department Of Molecular Genetics Kawasaki Medical School
-
Miyashita Naoyuki
Department Of General Internal Medicine 1 Kawasaki Hospital Kawasaki Medical School
-
Nishie-fujita Makiko
Department Of General Internal Medicine 1 Kawasaki Hospital Kawasaki Medical School
関連論文
- Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation
- Seroprevalence of Antibodies to Chlamydia spp.in Human Immunodeficiency Virus-Infected Subjects in Japan
- Continuous Isolation and Characterization of Chlamydia pneumoniae from a Patient with Diffuse Panbronchiolitis
- Isolation and Characterization of a Glycosylation Mutant from Schizosaccharomyces pombe
- Isolation and Characterization of a Novel Endo-β-galactofuranosidase from Bacillus sp.
- Evaluation of clinical dosage of gatifloxacin for respiratory tract infections in elderly patients based on pharmacokinetics/pharmacodynamics (PK/PD)
- Macrolide-resistant Mycoplasma pneumoniae in patients with community-acquired pneumonia
- Influence of gatifloxacin, a new quinolone antibacterial, on pharmacokinetics of theophylline
- Pulmonary Alveolar Proteinosis with Bilateral Ground-glass Opacities Localized in Subpleural Areas
- Evaluation of the diagnostic usefulness of real-time PCR for detection of Chlamydophila pneumoniae in acute respiratory infections
- Pulmonary Mycobacterium intracellulare disease with a solitary pulmonary nodule detected at the onset of pneumothorax
- Lung Adenocarcinoma Required the Differentiation from Wegener Granulomatosis
- Association of the RIP2 Gene with Childhood Atopic Asthma
- Association between genetic variation in the gene for death-associated protein-3 (DAP3) and adult asthma
- An association study of asthma and related phenotypes with polymorphisms in negative regulator molecules of the TLR signaling pathway
- Relationship between clinical efficacy of treatment of pulmonary Mycobacterium avium complex disease and drug-sensitivity testing of Mycobacterium avium complex isolates
- Infectious bulla of the lung caused by Mycobacterium intracellulare
- Hermansky-Pudlak Syndrome with Interstitial Pneumonia without Mutation of HSP1 Gene
- Multifocal Micronodular Pneumocyte Hyperplasia in a Man with Tuberous Sclerosis
- Chronic Necrotizing Pulmonary Aspergillosis Complicated by a Cavitary Lesion Caused by Pulmonary Mycobacterium-avium Complex Disease
- Pulmonary Mycobacterium Avium Disease with a Solitary Pulmonary Nodule Requiring Differentiation from Recurrence of Pulmonary Adenocarcinoma
- Thoracic actinomycosis with mainly pleural involvement
- Effect of pazufloxacin mesilate on the serum concentration of theophylline
- The 7.5-kb common plasmid is unrelated to the drug susceptiility of Chlamydia trachomatis
- Serum KL-6 as a Possible Marker for Amiodarone-induced Pulmonary Toxicity
- Association of Chronic Infection of Chlamydia pneumoniae and Coronary Heart Disease in the Japanese
- The effect of inhaled corticosteroids on Chlamydophila pneumoniae and Mycoplasma pneumoniae infection in children with bronchial asthma
- Clinical evaluation of QuantiFERON TB-2G test in patients with healed pulmonary tuberculosis
- An asymptomatic case of pulmonary cryptococcosis with endobronchial polypoid lesions and bilateral infiltrative shadow
- Clinical features of immunocompromised and nonimmunocompromised patients with pulmonary tuberculosis
- In Vitro Susceptibility of 7.5-kb Common Plasmid-Free Chlamydia trachomatis Strains
- Factor XIII in Henoch-Schonlein purpura with isolated gastrointestinal symptoms
- A Case of Mediastinal T-cell Lymphoblastic Lymphoma Causing Superior Mediastinal Syndrome and Severe Complications
- Clinical usefulness of QuantiFERON TB-2G test for the early diagnosis of pulmonary Mycobacterium kansasii disease
- N-acetylcysteine improves cytotoxic activity of cirrhotic rat liver-associated mononuclear cells
- Self-cloning Yeast Strains Containing Novel FAS2 Mutations Produce a Higher Amount of Ethyl Caproate in Japanese Sake
- Isolation of Five Laccase Gene Sequences from the White-Rot Fungus Trametes sanguinea by PCR, and Cloning, Characterization and Expression of the Laccase cDNA in Yeasts
- Detection of a Point Mutation in FAS2 Gene of Sake Yeast Strains by Allele-Specific PCR Amplification
- Construction of Recombinant Sake Yeast Containing a Dominant FAS2 Mutation without Extraneous Sequences by a Two-Step Gene Replacement Protocol
- Copper-Dependent Production of a Pycnoporus coccineus Extracellular Laccase in Aspergillus oryzae and Saccharomyces cerevisiae
- A Dominant Mutation Which Suppresses Deletion Mutations in the Secretory Signal Sequences of Glucoamylase from the Yeast Saccharomyces diastaticus(Biological Chemistry)
- Construction and Characterization of Mutant Glucoamylases from the Yeast Saccharomycopsis fibuligera(Biological Chemistry)
- Genes Required for Transcription of STA1 encoding an Extracellular Glucoamylase in the Yeast Saccharomyces(Biological Chemistry)
- Upstream Regions of the Yeast Glucoamylase Gene Which Are Required for Efficient Transcription(Biological Chemistry)
- Preventive Effect of a Traditional Herbal Medicine, Hochu-ekki-to, on Immunosuppression Induced by Surgical Stress
- Self-limiting Pneumonia Due to Chlamydia pneumoniae
- Isolation of a Novel Mutant Strain of Saccharomyces cerevisiae by an Ethyl Methane Sulfonate-Induced Mutagenesis Approach as a High Producer of Bioethanol(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effects of antiasthma drugs on the growth of Chlamydophila pneumoniae in HEp-2 cells
- Rapid diagnosis of adenovirus respiratory tract infections by immunochromatography
- Screening of Drugs That Suppress Ste11 MAPKKK Activation in Yeast Identified a c-Abl Tyrosine Kinase Inhibitor
- Development of a Novel Functional High-Throughput Screening System for Pathogen Effectors in the Yeast Saccharomyces cerevisiae
- Combinatorial Gene Overexpression and Recessive Mutant Gene Introduction in Sake Yeast
- In Vitro and In Vivo Activities of Sparfloxacin and Reference Drug Against Chlamydia pneumoniae
- Community-acquired Chlamydia pneumoniae Pneumonia in Japan : A Prospective Multicenter Community-acquired Pneumonia Study
- Recurrent Pneumonia due to Persistent Chiamydia Pneumoniae Infection
- The Relationship between Sperm Morphology and In Vitro Fertilization Ability in Mice
- Community-acquired macrolide-resistant Mycoplasma pneumoniae pneumonia in patients more than 18 years of age
- Results of a multicenter survey of diagnosis and treatment for bacterial meningitis in Japan
- A case report of measles in a premature infant and the antibody and/or the virus RNA in the patients contracted in infancy
- Cloning of a Gene Coding for Rhodotorucin A, a Farnesyl Peptide Mating Pheromone of Rhodosporidium toruloides(Biological Chemistry)
- Outbreak of Pertussis in a University Laboratory
- Q fever in adults patients with prolonged cough
- Microbiology of Chlamydiae : with Emphasis on Physiocochemistry, Antigenicity and Drug Susceptibility of Chlamydia pneumoniae
- Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation
- Recurrence of Sarcoidosis Following Interferon Alpha Therapy for Chronic Hepatitis C
- In-vitro activity of moxifloxacin and other fluoroquinolones against Chlamydia species
- Analysis of the serological response to Chlamydia pneumoniae in patients with ischemic heart disease by recombinant MOMP-ELISA
- Genetically Modified Industrial Yeast Ready for Application(BREWING AND FOOD TECHNOLOGY)
- Japanese Guidelines for the Management of Respiratory Infectious Diseases in Children 2007 with focus on pneumonia
- Evaluation of Serological Tests Detecting Chlamydophila pneumoniae-specific Immunoglobulin M Antibody
- Japanese guidelines for the management of community-acquired pneumonia : is it possible to distinguish between mycoplasmal pneumonia and bacterial pneumonia?
- Incidence of Penicillin-Resistant Streptococcus pneumoniae
- In Vitro Activities of 11 Antimicrobial Agents against Macrolide-Resistant Mycoplasma pneumoniae Isolates from Pediatric Patients: Results from a Multicenter Surveillance Study
- Clinical effect of 3g/day administration of meropenem on severe pneumonia
- Novel Small-Molecule Compounds That Affect Cellular Morphogenesis in Yeast and Mammalian Cells
- Clinical effect of sulbactam/ampicillin on community-acquired pneumonia with positive Streptococcus pneumoniae urinary antigen test
- Clinical Features and the Role of Atypical Pathogens in Nursing and Healthcare-associated Pneumonia (NHCAP): Differences between a Teaching University Hospital and a Community Hospital
- Discussion Regarding Tuberculin Skin Tests and Interferon-gamma Release Assays for the Differential Diagnosis of Pulmonary Tuberculosis
- A practical approach estimating etiologic agents using real-time PCR in pediatric inpatients with community-acquired pneumonia