Copper-Dependent Production of a Pycnoporus coccineus Extracellular Laccase in Aspergillus oryzae and Saccharomyces cerevisiae
スポンサーリンク
概要
- 論文の詳細を見る
Laccase is a multicopper-containing enzyme that catalyzes the oxidation of phenolic compounds. lcc1 cDNA coding for a secretory laccase of Pycnoporus coccineus was expressed under the maltose inducible amyB promoter in Aspergillus oryzae and under the galactose inducible GAL10 promoter in Saccharomyces cerevisiae. Laccase activities, which were undetectable in the absence of copper, were observed by increasing copper concentrations in the media for both systems. The amounts of secreted laccase protein but not lcc1 mRNA increased in proportion to copper concentrations in A. oryzae. The extracellular activities of native A. oryzae amylase and recombinant RNase-T1 expressed from the same amyB promoter in A. oryzae were constant regardless of copper concentrations. Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process.
- 社団法人 日本農芸化学会の論文
- 2005-06-23
著者
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HOSHIDA Hisashi
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine
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AKADA Rinji
Department of Applied Molecular Bioscience, Yamaguchi University Graduate School of Medicine
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Akada Rinji
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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MURATA Kenji
Department of Applied Physics, Faculty Engineering, Osaka University
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Kubo Kanako
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Hoshida Hisashi
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Murata Kenji
Department Of Animal Science University Of California
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Morimasa Koji
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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FUJITA Tsuneyasu
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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Fujita Tsuneyasu
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Kubo K
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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