Effect of pH on brdicka currents produced by cytochrome c.
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概要
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Effect of pH and buffer concentrations on Brdicka currents produced by horse heart cytochrome c was investigated in the presence of hexaamminecobalt(III) chloride in ammonia, tris(hydroxymethyl)aminomethane, <I>N</I>,<I>N</I>-dimethylglycine, borate, and carbonate buffer solutions at pH varying between 8.2 and 10.7. The Brdicka current-activity of the protein varied with pH and the kind and concentration of buffers, but, if corrected for the effect of the buffer salts, showed a sigmoidal pH-dependence. The pH-dependence was interpreted as due to the protonic dissociation of Lys-79 of the protein. Brdicka currents of the protein were observed also in unbuffered solutions containing no proton donor except water. The current intensity was explained by taking into account the change of pH at electrode surface. The results indicate that a main proton donor in the Brdicka reaction is a water molecule.
- 公益社団法人 日本化学会の論文
著者
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Ikeda Tokuji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Senda Mitsugi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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Kinoshita Hideaki
Department Of Agricultural Chemistry Kyoto University
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Yamane Yoshiyuki
Department Of Mechanical Engineering Tokyo Institute Of Technology
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Kinoshita Hideaki
Department of Chemistry, Kwassui Women's College
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