Polarographic Study on Interaction between HumanIgG and Sheep AntihumanIgG Antiserum and Its Analytical Application
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概要
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The Brdicka current (polarographic catalytic hydrogen evolution current produced by proteins in the presence of cobalt salt) of human immunogloblin G (IgG) and sheep antihuman IgG antiserum (anti-IgG) was studied by the d.c. and differential pulse polarographic techniques. The Brdicka current for a mixture ofIgG and anti-IgG was smaller than the sumof the currents for IgG and anti-IgG. This difference was attributed to the complex formation between IgG and anti-IgG. Anti-IgG could be titrated with IgG (or vice versa) by polarographic techniques based on the Brdicka current. The average intrinsic dissociation constant of the IgG-anti-IgG complexes was estimated from the titration curve. The polarographic methodbased on the Brdicka current and coupled with immunoreaction is useful to determine trace amounts of antigen (or antibody) down to 10-10 m using the differential pulse polarographic technique.
- 社団法人 日本農芸化学会の論文
著者
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IKEDA Tokuji
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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Kano Kenji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Ikeda Tokuji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Senda Mitsugi
Department Of Agricultural Chemistry College Of Agriculture Kyoto University
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