An Enzyme-Linked Immunosorbent Assay for Desmosine

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概要

• 論文の詳細を見る

• この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。A sensitive, reproducible and rapid analytical method that is capable of identifying the presence of lung damage at an early stage is required. We have developed a sensitive enzyme-linked immunosorbent assay for desmosine in human body fluids. An amino group of desmosine was exclusively linked to bovine gamma globulin and the high-titer antibody to this conjugate was prepared. This antibody was used in this assay. This method enables the routine analysis of large numbers of samples with a detection limit of 0.4ng desmosine. Using this method, we quantitatively measured desmosine in the urine of 8 normal controls and 25 patients with chronic obstructive lung disease. The results were expressed as desmosine/creatine ratio. On average, a statistically significant higher level of desmosine/creatinine was found in the patients compared to the controls.

• 京都大学の論文
• 1986-08-31

著者

• MATSUMOTO Takashi

  Central Research Institute, Japan Tobacco Inc.

• Mizusaki S

  Toyotama Healthy Food Co. Ltd. Tokyo Jpn

• Mizusaki Shigenobu

  Central Research Institute Japan Tobacco Inc.

• NISHIMURA Koichi

  Chest Disease Research Institute, Kyoto University

• OSHIMA Shunsaku

  Chest Disease Research Institute, Kyoto University

• Oshima Shunsaku

  Chest Disease Research Institute Kyoto University

• Nishimura Koichi

  Chest Disease Research Institute Kyoto University

• Matsumoto Takashi

  Central Research Institute Japan Tobacco Inc.

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