Glycine as a substrate for photorespiration

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概要

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• Substrates for photorespiration were examined by feeding ^<14>C labeled compounds to tobacco and corn leaf segments and by measuring ^<14>CO_2 evolution in light and darkness. CO_2 release in the dark was rapid, but in light CO_2 release was slow due to refixation by photosynthesis. Carboxyl labeled glycine was more rapidly decarboxylated than were glyoxylate, glycolate or serine. Hydroxypyridinemethane sulfonate, an inhibitor of glycolate oxidase, blocked CO_2 release from glycolate but not from glycine. Isonicotynyl hydrazide blocked CO_2 release from both glycine and glycolate. DCMU blocked photosynthetic refixation of the released CO_2, consequently the rates of CO_2 release in light and dark were about equal. It was concluded that CO_2 release during photo-respiration came from the conversion of 2 molecules of glycine to one serine and one CO_2. ^<14>CO_2 release from glycine-1-^<14>C in the dark or with DCMU in light can be used as an assay for photorespiration ability. CO_2 release from glycine and glycolate by corn leaf segments in the dark proceeded at 2/3 the rate of that in normal tobacco leaf. This result, together with other work on O_2 exchange and enzymatic analysis, indicates that corn and other plants do carry on photorespiration, but it is not manifested by CO_2 release in light. A yellow tobacco mutant, Consolation 402, had high rates of photorespiration by the ^<14>CO_2 assay, nearly half (or more) as many peroxisomes as chloroplasts, and high rates of CO_2 release from glycine-1-^<14>C or glycolate-1-^<14>C. A common tobacco, Bright Yellow, had lower rates of photorespiration, fewer visible peroxisomes, and slower decarboxylation of glycine and glycolate. The amount of ^<14>CO_2 release from glycine-1-^<14>C or glycolate-1-^<14>C increased only slightly when the temperature was raised from 25 to 35℃.

• 日本植物生理学会の論文

著者

• Kisaki Takuro

  Central Research Institute Japan Monopoly Corporation

• Tolbert N.e.

  Central Research Institute Japan Monopoly Corporation

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