Purification and Characterization of Thermostable Pyrimidine Nucleoside Phosphorylase from Bacillus stearothermophilus JTS 859(Biological Chemistry)
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概要
- 論文の詳細を見る
A thermostable pyrimidine nucleoside phosphorylase has been purified from Bacillus stearothermophilus JTS 859. The enzyme had a molecular weight of 85,000, consisting of 2 identical subunits (M_w 54,000). Its isoelectric point was 4.8. The Michaelis constants for 5-methyluridine, uridine, thymidine, and 2'-deoxyuridine were 0.32, 0.19, 0.46, and 0.58mM, respectively. The optimal temperature of the reaction was 70℃. The enzyme was found not to be a SH-enzyme based on the following three points. First, the enzyme reaction was not inhibited by PCMB or iodoacetic acid. Second, the optimal pH of the reaction was broad, from 7.0 to 11.5. Third, the enzyme did not contain cysteine. The half-life of the enzyme was 15.1 hr in 20mM potassium phosphate and 1mM 5-methyluridine (pH 7.0) at 70℃. Due to the high optimal temperature, the broad optimal pH range, and the long half-life of the enzyme, the enzyme is useful for practical applications.
- 社団法人日本農芸化学会の論文
- 1990-03-23
著者
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Hori N
Tobacco Science Research Laboratory
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Hori Nobuaki
Central Research Institute Japan Tobacco Inc.
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Mikami Y
Chiba Univ. Chiba Jpn
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Watanabe M
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Hori Nobuaki
Tobacco Science Research Laboratory Japan Tobacco Inc.
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WATANABE Mutsumi
Tobacco Science Research Laboratory, Japan Tobacco Inc.
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YAMAZAKI Yoshinari
Tobacco Science Research Laboratory, Japan Tobacco Inc.
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MIKAMI Yoichi
Tobacco Science Research Laboratory, Japan Tobacco Inc.
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Watanabe M
Graduate School Of Engineering Hiroshima Kokusai Gakuin University
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Mikami Yoichi
Tobacco Science Research Laboratory
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Mikami Yoichi
Central Research Institute The Japan Tobacco 〓 Salt Public Corporation
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Hori Nobuaki
Tobacco Science Research Laboratory
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Yamazaki Y
Agency Of Industrial Science And Technology Ibaraki Jpn
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Mikami Y
Tokyo Laboratory Yuki Gosei Kogyo Co. Ltd.
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