Characterization of a PDR1 Mutant Allele from a Clotrimazole-Resistant Saka Yeast Mutant with Improved Fermentative Activity
スポンサーリンク
概要
- 論文の詳細を見る
Clotrimazole-resistant mutants from various sake yeasts show improved fermentative activity in sake mash while retaining their parental advantages for sake making. These mutants also exhibit pleiotropic drug resistance(PDR)phenotypes. To investigate the relationship between the improvement of fermentative activity and PDR phenotypes, a PDR1 mutant allele(pdr1-h176)encoding a transcription factor was cloned from a clotrimazole-resistant mutant, HL176(MATa/MATα), using PCR amplification. The nucleotide sequences of pdr1-h176 and its wild allele were determined. The mutant allele contained a missense point mutation(L309S)that can confer a PDR phenotype on yeast. this amino acid substitution is located in the conserved motif II in the inhibitory domain of Pdr1p, and is very close to the cluster of three mutation points(P298A, K302Q, and M308I)described by Carvajal et al.(Mol.Gen.Genet., 256, 406-415, 1997)in laboratory strains. A PDR1 wild allele of HL163, the parent strain of HL176, was replaced by pdr1-h176 using gene recombination at the homologous site. The resultant transformants(PDR1/pdr1-h176)showed the same PDR phenotype as HL176, and they fermented sake mash efficiently even in the final fermentation stage, while HL163 did not. The amino acid substitution(L309S)in pdr1-h176 was considered to be sufficient to improve the fermentative activity of sake yeast, in addition to conferring the PDR phenotype.
- 社団法人日本生物工学会の論文
- 1999-07-25
著者
-
Watanabe M
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Nishimura Akira
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
-
Nishimura A
Tokyo Univ. Chiba Jpn
-
Watanabe M
Graduate School Of Engineering Hiroshima Kokusai Gakuin University
-
WATANABE MUTSUMI
Research Laboratory, Hakutsuru Sake Brewing Co. Ltd.
-
MIZOGUCHI HIROKO
Research & Development Department, Hakutsuru Sake Brewing Co. Ltd.
-
Mizoguchi Haruhiko
General Research Laboratory of Kiku-Masamune Sake Brewing Co., Ltd.
-
Mizoguchi H
General Research Laboratory Of Kiku-masamune Sake Brewing Co. Ltd.
-
Mizoguchi Haruhiko
General Research Laboratory Kiku-masamune Sake Brewing Co. Ltd.
-
Watanabe Mutsumi
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
-
Nishimura Akira
Research & Development Department Hakutsuru Sake Brewing Co. Ltd
関連論文
- Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Effects of Aldehyde Dehydrogenase and Acetyl-CoA Synthetase on Acetate Formation in Sake Mash
- Purification and Characterization of Thermostable Pyrimidine Nucleoside Phosphorylase from Bacillus stearothermophilus JTS 859(Biological Chemistry)
- Purification and Characterization of Second Thermostable Purine Nucleoside Phosphorylase in Bacillus stearothermophilus JTS 859(Biological Chemistry)
- Molecular Breeding of a Sake Yeast with a Mutated ARO4 Gene Which Causes Both Resistance to o-Fluoro-DL-Phenylalanine and Increased Production of β-Phenethyl Alcohol
- Altered Regulation of Aromatic Amino Acid Biosynthesis in β-Phenylethyl-alcohol-overproducing Mutants of Sake Yeast Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- New Insertion Sequence in Lactobacillus fructivorans Strains Isolated from Spoiled Sake(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Applications of Photosynthetic Bacteria for Medical Fields
- Biosorption of Cadmium Ions Using a Photosynthetic Bacterium,Rhodobacter sphaeroides S and a Marine Photosynthetic Bacterium,Rhodovulum sp. and Their Biosorption Kinetics
- Removal of Phosphorus from Oyster Farm Mud Sediment Using a Photosynthetic Bacterium, Rhodobacter sphaeroides IL106
- Flocculating Property of Extracellular Polymeric Substance Derived from a Marine Photosynthetic Bacterium, Rhodovulum sp.
- Treatment of Aquarium Water by Denitrifying Photosynthetic Bacteria Using Immobilized Polyvinyl Alcohol Beads
- Properties of Sake Yeast Mutants Resistant to Isoamyl Monochloroacetate
- Identification of Sialyltransferases of Streptococcus agalactiae
- Different Missense Mutations in PDR1 and PDR3 Genes from Clotrimazole-Resistant Sake Yeast are Responsible for Pleiotropic Drug Resistance and Improved Fermentative Activity
- Molecular Characterization of a Novel β1, 3-Galactosyltransferase for Capsular Polysaccharide Synthesis by Streptococcus agalactiae Type Ib^1
- Putative Regulatory Gene for Polysaccharide Synthesis of Streptococcus agalactiae Type Ia
- Disruption of the ABC Transporter Genes PDR5, YOR1, and SNQ2, and Their Participation in Improved Fermentative Activity of a Sake Yeast Mutant Showing Pleiotropic Drug Resistance
- Characterization of a PDR1 Mutant Allele from a Clotrimazole-Resistant Saka Yeast Mutant with Improved Fermentative Activity
- Screening of Bacteriophages Producing Endo-N-Acetylneuraminidase
- Mutants Producing High Concentrations of the Flavor Components Active Amylalcohol and Normal Propanol in Saccharomyces cerevisiae
- Optimal Culture Conditions of Bacillus stearothermophilus JTS 859 for the Production of a Thermostable Enzyme to Synthesize 5-Methyluridine
- Mutants of Yeast Saccharomyces cerevisiae Producing Large Amounts of the Flavor Components Isobutyl Alcohol and Isoamyl Alcohol(Microbiology & Fermentation Industry)
- Breeding of Brewing Yeast Producing a Large Amount of β-Phenylethyl Alcohol and β-Phenylethyl Acetate(Microbiology & Fermentation Industry)
- Absolute cues for auditory distance in front and lateral directions
- Molecular Breeding of the Mureka-Non-Forming Sake Koji Mold from Aspergillus oryzae by the Disruption of the mreA Gene(BREWING AND FOOD TECHNOLOGY)
- Transformation of Aspergillus sp. and Trichoderma reesei Using the Pyrithiamine Resistance Gene (ptrA) of Aspergillus oryzae(Biochemistry & Molecular Biology)
- Pyrithiamin Resistance Gene (ptrA) of Aspergillus oryzae : Cloning, Characterization and Application as a Dominant Selectable Marker for Transformation
- Molecular Cloning of the Isoamyl Alcohol Oxidase-Encoding Gene(mreA)from Aspergillus oryzae
- Purification and Characterization of Isoamyl Alcohol Oxidase ("Mureka"-Forming Enzyme)
- Isolation of Copper-Tolerant Mutants of Sake Yeast with Defective Peptide Uptake(BREWING AND FOOD TECHNOLOGY)
- Effect of Amino Acids on Peptide Transport in Sake Yeast(Brewing and Food Technology)
- Effect of Cellular Inositol Content on Ethanol Tolerance of Saccharomyces cerevisiae in Sake Brewing(BREWING AND FOOD TECHNOLOGY)
- Increased Alcohol Acetyltransferase Activity by Inositol Limitation in Saccharomyces cerevisiae in Sake Mash
- Increased Ethyl Caproate Production by Inositol Limitation in Saccharomyces cerevisiae
- Effect of Fatty Acid Saturation in Membrane Lipid Bilayers on Simple Diffusion in the Presence of Ethanol at High Concentrations
- Differences in the Intracellular Lipids of Sake Yeast in Main Mash Seeded Respectively with Two Kinds of Seed Mash : Kimoto and Sokujo-moto
- Isolation and Characterization of Sake Yeast Mutants Deficient in γ-Aminobutyric Acid Utilization in Sake Brewing(BREWING AND FOOD TECHNOLOGY)
- High Fidelity Segregation of a YEp Vector in [cir^0] Strains of the Yeast Saccharomyces cerevisiae
- A Role of Saccharomyces cerevisiae Fatty Acid Activation Protein 4 in Palmitoyl-CoA Pool for Growth in the Presence of Ethanol
- The Promoter Activity of Isovaleryl-CoA Dehydrogenase-Encoding Gene (ivdA) from Aspergillus oryzae Is Strictly Repressed by Glutamic Acid
- Bioethanol production from rice washing drainage and rice bran(BIOCHEMICAL ENGINEERING)
- Construction of a thiamine pyrophosphate high-producing strain of Aspergillus oryzae by overexpression of three genes involved in thiamine biosynthesis(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Isolation of Sake Yeast Mutants Resistant to Isolamyl Monofluoroacetate to Improve Isoamyl Acetate Productivity
- Effect of enzymatic treatment on sedimentation and flocculation abilities of solid particles in rice washing drainage and its relationship with protein profiles(BIOCHEMICAL ENGINEERING)
- High-yields heterologous production of the novel Aspergillus fumigatus elastase inhibitor AFUEI in Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- Sake Yeast Mutants with Improved Fermentative Activity: Isolation, Application, and a Novel Mechanism
- Rational Design of a New IMP Aptamer Based on a TPP Riboswitch and a Hypoxanthine Aptamer