Properties of Sake Yeast Mutants Resistant to Isoamyl Monochloroacetate
スポンサーリンク
概要
- 論文の詳細を見る
Isoamyl monochloroacetate (i-AmOClAc) inhibited the growth of sake yeast. AOF10,which is resistant to isoamyl monofluoroacetate and has low esterase activity toward isoamyl acetate (i-AmOAc), also showed resistance to i-AmOClAc. Monochloroacetic acid formed by an esterase through the hydrolysis of i-AmOClAc was more effective at glycolysis inhibition than i-AmOClAc. Forty five mutants resistant to i-AmOClAc were isolated from a parental haploid strain (HL-69) derived from sake yeast. Three had less than 20% of the original activity of the esterase. All mutants fermented sake mash as well as the parent strain, HL-69,and accumulated 1.3 to 1.7-fold the levels of i-AmOAc and 1.8 to 2.4-fold the levels of isobutyl acetate accumulated by HL-69. Surprisingly one mutant (CL-90), which accumulated 1.7-fold the amount of i-AmOAc and 1.3-fold the amount of ethyl acetate, exhibited increased alcohol acetyltransferase (EC 2.3.1.84) activity (1.6-fold). Esterase patterns were also examined by gel electrophoresis and activety staining. Changes in the intensity of the strongest band (EST2) were closely related to the activity of the esterase toward i-AmOAc.
- 社団法人日本生物工学会の論文
- 1995-09-25
著者
-
WATANABE MUTSUMI
Research Laboratory, Hakutsuru Sake Brewing Co. Ltd.
-
NAGAI HIDEO
Research Laboratory, Hakutsuru Sake Brewing Co. Led.
-
KONDO KYOICHI
Research Laboratory, Hakutsuru Sake Brewing Co. Ltd.
-
Nagai Hideo
Research Laboratory Hakutsuru Sake Brewing Co. Led.
-
Watanabe Mutsumi
Research And Development Department Hakutsuru Sake Brewing Co. Ltd.
関連論文
- Properties of Sake Yeast Mutants Resistant to Isoamyl Monochloroacetate
- Different Missense Mutations in PDR1 and PDR3 Genes from Clotrimazole-Resistant Sake Yeast are Responsible for Pleiotropic Drug Resistance and Improved Fermentative Activity
- Disruption of the ABC Transporter Genes PDR5, YOR1, and SNQ2, and Their Participation in Improved Fermentative Activity of a Sake Yeast Mutant Showing Pleiotropic Drug Resistance
- Characterization of a PDR1 Mutant Allele from a Clotrimazole-Resistant Saka Yeast Mutant with Improved Fermentative Activity
- Isolation of Sake Yeast Mutants Resistant to Isolamyl Monofluoroacetate to Improve Isoamyl Acetate Productivity
- Sake Yeast Mutants with Improved Fermentative Activity: Isolation, Application, and a Novel Mechanism