Enzymatic Synthesis of Optically Pure (R)-(-)-Mandelic Acid and Other 2-Hydroxycarboxylic Acids: Screening for the Enzyme, and Its Purification, Characterization and Use(Biological Chemistry)
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概要
- 論文の詳細を見る
An enzyme that reduces benzoylformate with NADH to form (R)-mandelate was extracted from cells of Streptococcus faecalis IFO 12964 and purified to more than 95% purity as evidenced by gel electrophoresis. Physicochemical and enzymic properties were studied. From the substrate specificity, we concluded that the enzyme was a kind of (R)-2-hydroxyisocaproate dehydrogenase. Optically pure (R)-(-)-mandelic acid was prepared with the enzyme, NADH, and alcohol, formate or glucose dehydrogenase in 84〜93% yield. Five (R)-2-hydroxyalkanoic acids (C_4〜C_6) or their Ba salts, (R)-(+)-3-phenyllactic acid and (S)-(-)-3-chlorolactic acid were also prepared with the enzyme.
- 社団法人日本農芸化学会の論文
- 1986-10-23
著者
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Maeda H
Wakayama Agricultural Biological Inst. Center Wakayama Jpn
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Yamazaki Y
Agency Of Industrial Science And Technology Ibaraki Jpn
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YAMAZAKI Yoshimitsu
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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MAEDA Hidekatsu
Fermentation Research Institute, Agency of Industrial Science and Technology, Ministry of Internatio
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Maeda Hidekatsu
Fermentation Research Institute Agency Of Industrial Science And Technology
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Yamazaki Yoshimitsu
Fermentation Research Institute Agency Of Industrial Science And Technology
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