The Synthesis of New Polymer Derivatives of NAD by Radical Copolymerization and Their Coenzymic Activity

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概要

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• Three polymerizable NAD derivatives, N6-[N-(6-methacrylamidohexyl)carbamoylmethyl]-, N6-[N-[2-[N-(2-methacrylamidoethyl)carbamoyl]ethyl]carbamoylmethyl]-, and N6-[N-[N-(2-hydroxy-3-methacrylamidopropyl)carbamoylmethyl]carbamoylmethyl] -NAD, were synthesized and radically copolymerized with various comonomers [acrylamide, N-(2-hydroxyethyl)-, N-ethyl-, N, N-diethyl-, and N, N-dimethylacrylamide, acrylic acid, and 6-methacrylamidohexylammonium chloride] to give 21 new polymer derivatives of NAD. The monomeric and polymeric NAD derivatives were all coenzymically active against alcohol, lactate, and malate dehydrogenase. The coenzymic activities were affected most strongly by the chemical property of the spacer, accompanied by a regular decrease in Km versus increasing spacer hydrophobicity. The chemical property of the polymer body also affected the coenzymic activities; the hydrophobic polymer bodies (copolymers with N, N-diethyl- and N, N-dimethylacrylamide) were efficient as NAD carriers for lactate and malate dehydrogenase, and the positively charged one (copolymer with acrylamide plus 6-methacrylamidohexylammonium chloride) led to good coenzymic activities of the polymeric NAD derivative against all three dehydrogenases. The effects of the NAD density and average molecular weight of the polymer derivatives were also tested.

• 社団法人 日本農芸化学会の論文

著者

• Maeda Hidekatsu

  Fermentation Research Institute Agency Of Industrial Science And Technology

• Yamazaki Yoshimitsu

  Fermentation Research Institute Agency Of Industrial Science And Technology

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