The Co-immobilization of NAD and Dehydrogenases and Its Application to Bioreactors for Synthesis and Analysis

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概要

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• A polymerizable NAD derivative, N6-[N-[N-(2-hydroxy-3-methacrylamidopropyl)carbamoylmethyl]carbamoylmethyl]-NAD, formate dehydrogenase, and malate dehydrogenase were entrapped all together in polyacrylamide gels. The entrapment was carried out by radical copolymerization, and consequently NAD was bound on the matrix which enclosed the enzymes. These gels had the function of producing L-malate from oxalacetate and formate. The L-malate production was also continuously done in a column reactor for 3 days. Another gel was similarly prepared with N6-[N-(6-methacrylamidohexyl)carbamoylmethyl]-NAD, horse liver alcohol dehydrogenase, and diaphorase. This gel was shown to catalyze the formation of resorufin from resazurin and ethanol. This gel was applicable to ethanol analysis using a fluorescence spectrophotometer to determine resorufin. The analyzer was usable for one week.

• 社団法人 日本農芸化学会の論文

著者

• Maeda Hidekatsu

  Fermentation Research Institute Agency Of Industrial Science And Technology

• Yamazaki Yoshimitsu

  Fermentation Research Institute Agency Of Industrial Science And Technology

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