Purification and Characterization of a NAD^+-Dependent Formate Dehydrogenase Produced by Paracoccus sp.
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概要
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A facultative formate-utilizing bacterium, Paracoccus sp. strain 12-A, with a high specific activity (11.6 units/mg) of NAD^+-dependent formate dehydrogenase, was isolated from sewage. From a crude extract of the cells, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-cellulose column chromatography and hydroxyapatite column chromatography. The enzyme protein amounted to 15% of intracellular soluble proteins in strain 12-A. The enzyme had a molecular weight of approximately 100,000 by gel filtration with Sephadex G-150 and 49,000 by SDS-polyacrylamide gel electrophoresis, and an isoelectric point of 5.4. The pH and temperature optima ware 6.5-7.5 abd 50-55℃, respectively. The enzyme was stable at pH 6.0-10.0 and up to 50℃. The apparent K_m values for formate and NAD^+ were calculated to be 5.0 mM and 0.036 mM, respectively.
- 社団法人日本農芸化学会の論文
- 1992-12-23
著者
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Iida Mitsugi
Department Of Applied Biological Science Faculty Of Science And Technology Science Niversity Of Toky
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Mineki Shigeru
Department Of Applied Biological Science Faculty Of Sciecne And Technology Science University Of Tok
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Maeda Hidekatsu
Fermentation Research Institute Agency Of Industrial Science And Technology
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Iida Mitsugi
Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok
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KITAMURA-KIMURA Keiko
Department of Applied Biological Science, Faculty of Science and Technology, Science University of T
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Mineki Shigeru
Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok
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Kitamura-kimura Keiko
Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok
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