Some New Intermediates in Microbial Side Chain Degradation of β-Sitosterol : Studies on Microbial Transformation (XXVII).
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概要
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By preselection of microorganisms which preferentially attach the side chain of β-sitosterol, we isolated a mutant H-45 of Rhodococcus equi K-3 which produces stigmasta-1,4-dien-3-on-26-oic acid from β-sitosterol in a high yield. The mutant produced new compounds such as ergosta-1,4-dien-3-on-26-oic acid, stigmast-4-en-3-on-26-oic acid, and ergost-4-en-3-on-26-oci acid, as well as the known β-sitosta-1,4-dien-3-one, β-sitost-4-en-3-one, 20-carboxy-pregn-4-en-3-one, 20-carboxy-pregna-1,4-dien-3-one, androst-4-en-3,17-dione, androsta-1,4-dien-3,17-dione, propionic acid, and acetic acid. The structures of these products were established by Mass and ^1H-NMR spectroscopy. From microbial transformation of the 25-carboxylic acids obtained, we concluded that stigmasta-1,4-dien-3-on-26-oic acid is a hypothetical intermediate in β-sitosterol side chain degradation. We propose a degradation pathway of the β-sitosterol side chain by Rhodococcus equi.
- 1993-09-25
著者
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Iida Mitsugi
Department Of Applied Biological Science Faculty Of Science And Technology Science Niversity Of Toky
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Murohisa Tomoo
Department Of Applied Biological Science Science University Of Tokyo
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