Bulk Preparation of R-(+)-Nicotine from a Racemic Mixture by Continuous Culture with an Isolated Pseudomonas putida
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概要
- 論文の詳細を見る
A bulk-preparative method for pure R-(+)-nicotine (1b) involving the complete stereospecific degradation of S-(-)-nicotine (1a) from a racemic nicorine (1ab) with Pseudomonas putida JTS-9 isolated from tobacco field soil was studied as regards a continuous culture system. The test of growth tolerance of the bacteria of 1a and 1b in the culture medium showed that 0.2% was critical for 1a and 0.8% for 1b. Under these limitations the continuous preparation of 1b with automatic feeding of 1% 1ab was stably achieved by sensing DO (3 ppm) using two jar fermentors.
- 社団法人日本生物工学会の論文
- 1983-06-25
著者
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Mikami Y
Chiba Univ. Chiba Jpn
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Maeda Susumu
The Central Research Institute Japan Tobacco And Sdlt Public Corporation
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Obi Yukiteru
The Central Research Institute Japan Tobacco And Sdlt Public Corporation
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Kisaki Takuro
The Central Research Institute Japan Tobacco And Sdlt Public Corporation
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Kisaki Takuro
The Central Research Institute Japan Monopoly Corporation
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Mikami Yoichi
Tobacco Science Research Laboratory
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Mikami Yoichi
The Central Research Institute Japan Tobacco And Salt Public Corporation
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Mikami Yoichi
Central Research Institute The Japan Tobacco 〓 Salt Public Corporation
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KATSUYAMA AKIO
The Central Research Institute, Japan Tobacco and Sdlt Public Corporation
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UCHIDA SETSUKO
The Central Research Institute, Japan Tobacco and Sdlt Public Corporation
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Mikami Y
Tokyo Laboratory Yuki Gosei Kogyo Co. Ltd.
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Katsuyama Akio
The Central Research Institute Japan Tobacco And Sdlt Public Corporation
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Uchida Setsuko
The Central Research Institute Japan Tobacco And Sdlt Public Corporation
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