Preparation and Application of Trichoderma reesei Mutants with Enhanced β-Glucosidase(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
Mutants with enhanced β-glucosidase production were isolated by the plate method with 4-methylumbelliferyl-β-D-glucoside as a substrate of β-glucosidase in Trichoderma reesei. Among them, the mutant strain CDU-11 had the highest production of β-glucosidase. In 5-liter jar fermentor culture of CDU-11 with 6% Avicel as a carbon source, β-glucosidase production was significantly increased by keeping the pH at 4.0 during the first 3 days, then keeping the pH at 5.5 for 4 days. CMCase reached 320 U/ml, Avicelase 29 U/ml, and β-glucosidase 21 U/ml. The ratio of β-glucosidase to CMCase in the CDU-11 culture was about three times that in KY 746, the parent strain. The saccharification of Avicel and alkali-treated bagasse by the cellulolytic system of CDU-11 became much more effective than with other strains.
- 社団法人日本農芸化学会の論文
- 1986-10-23
著者
-
Takasawa S
Foods And Liquors Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Takasawa Seigo
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
KAWAMORI Mikio
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
-
ADO Yutaka
Technical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
-
Ado Yutaka
Technical Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Kawamori M
Kyowa Hakko Kogyo Co. Ltd. Tokyo Jpn
-
Kawamori Mikio
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
関連論文
- Molecular Breeding of a Sake Yeast with a Mutated ARO4 Gene Which Causes Both Resistance to o-Fluoro-DL-Phenylalanine and Increased Production of β-Phenethyl Alcohol
- Mutants Producing High Concentrations of the Flavor Components Active Amylalcohol and Normal Propanol in Saccharomyces cerevisiae
- Production of Cellulases by a Thermophilic Fungus, Thermoascus aurantiacus A-131(Microbiology & Fermentation Industry)
- Feedback-Insensitive Mutation of 3-Deoxy-d-Arabino-Hepturosonate-7-Phosphate Synthase Caused by a Single Nucleotide Substitution of ARO4 Structural Gene in Saccharomyces cerevisiae
- Preparation and Application of Trichoderma reesei Mutants with Enhanced β-Glucosidase(Microbiology & Fermentation Industry)
- Enzymatic Production of Amoxicillin by β-Lactamase-deficient Mutants of Pseudomonas melanogenum KY 3987