Chromosome Bisection in the Yeast Saccharomyces cerevisiae Facilitated by Yeast Artificial Chromosomes Bearing a Site-specific Recombination System
スポンサーリンク
概要
- 論文の詳細を見る
A chromosome bisection method was constructed using yeast artificial chromosomes (YAC) and a GAL1-promoted site-specific recombination system. This method was applied to bisect chromosome IV into the left and right parts at the centromere region. The bisection occurred at frequencies of about 10% when the recombination site DNAs were integrated onto YAC and chromosome IV in the same direction, but were less than 10^<-3> when they were in opposite directions. Reconstruction of the original chromosome IV from the bisected chromosomes was also induced by galactose at high frequencies. Loss of the left part chromosome was found at the frequencies of 0.9×10^<-3> after hybrid cells between the chromosome-bisected strain and a normal haploid were subcultivated in a complete medium. The bisection and reconstruction of chromosome IV and deletion of the left part chromosome were demonstrated by electrophoretical karyotyping.
- 社団法人日本農芸化学会の論文
- 1995-09-23
著者
-
Ouchi Kozo
Tokyo Resarch Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Ouchi Kozo
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
-
Kawasaki Hideki
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
-
Kawasaki Hideki
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
関連論文
- Factors Affecting the Ethanol Productivity of Yeast in Molasses
- Molecular Breeding of a Sake Yeast with a Mutated ARO4 Gene Which Causes Both Resistance to o-Fluoro-DL-Phenylalanine and Increased Production of β-Phenethyl Alcohol
- Chromosome Bisection in the Yeast Saccharomyces cerevisiae Facilitated by Yeast Artificial Chromosomes Bearing a Site-specific Recombination System
- Improvement of Freeze Tolerance of Commercial Baker's Yeasts in Dough by Heat Treatment before Freezing
- Feedback-Insensitive Mutation of 3-Deoxy-d-Arabino-Hepturosonate-7-Phosphate Synthase Caused by a Single Nucleotide Substitution of ARO4 Structural Gene in Saccharomyces cerevisiae
- Effects of Yeast Invertase on Ethanol Production in Molasses
- A DNA Construct Useful for Specific Chromosome Loss in Saccharomyces cerevisiae
- Reconstruction of Ethanol Fermentation in Permeabilized Cells of the Yeast Saccharomyces cerevisiae